目的分析第四代HIV诊断试剂检测HIV抗体假阳性的原因，减少假阳性检出率。方法选取119例HIV四代试剂检测初筛阳性的标本，送HIV确认实验室确认HIV抗体结果，分析比对两者的结果，探讨出现假阳性的原因。结果119例HIV抗体初筛阳性的标本，送至确认实验室回报结果40例HIV抗体阳性，28例HIV抗体不确定，51例HIV抗体阴性。随着S/CO值的增高，初筛结果与确证结果阳性符合率也随之升高。结论HIV四代检测试剂假阳性率较高，这与试剂同时检测HIV抗体和P24抗原有关。当1<S/CO≤10时，实验室检测人员应依据实验检测结果，结合受检者相应病史和个体状况进行综合分析。
Abstract
Objective To study the causes of false positive results in the fourth generation p24 Ag/anti-HIV combined assay and cut down the false positive results. Methods We picked 119 samples which were reactive in HIV-Ab screening test with the fourth generation HIV assay, then confirmed these samples with West-blot assay and compared them. Results In 119 reactive samples, 40 samples were HIV-1 positive, 28 samples were HIV-Ab indeterminate, and 51 samples were HIV-Ab negative. The higher the S/CO index, the closer the positive results of screening test related to one of confirmatory test. Conclusions The rate of false positive is high with the fourth generation HIV assay, which is relative to the combined detection of anti-HIV-P24. When 1<S/CO≤10, we should consider comprehensive factors such as history of disease, the clinical symptoms, etc.
关键词
HIV抗体 /
HIV-1P24抗原 /
HIV四代试剂 /
假阳性反应
Key words
antibody to HIV /
HIV-1 P24 antigen /
the fourth generation HIV assay /
false positive reaction
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参考文献
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