磷酸盐法提取杏仁蛋白的工艺研究

刘凯,梁俊玉,肖引,陈江,陈芳

武警医学 ›› 2013, Vol. 24 ›› Issue (9) : 783-785.

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PDF(571 KB)
武警医学 ›› 2013, Vol. 24 ›› Issue (9) : 783-785.
论著

磷酸盐法提取杏仁蛋白的工艺研究

  • 刘凯1,梁俊玉2,肖引1,陈江1,陈芳1
作者信息 +

Extracting process conditions from almond dregs by phosphate method

  • LIU Kai1,LIANG Junyu2,XIAO Yin1,CHEN Jiang1,and CHEN Fang1 .
Author information +
文章历史 +

摘要

目的探讨以杏仁渣为原料提取杏仁蛋白的最优工艺条件。方法通过设计单因素实验和正交实验,将粉碎的杏仁渣加入磷酸盐溶液中水浴加热搅拌,离心,测定上清液的吸光度,计算提取率,确定提取工艺。结果采用磷酸盐法提取杏仁蛋白的工艺为,温度50℃、料液比1∶30,提取1.0h,盐浓度为0.01mol/L,蛋白质提取率为72%。结论采用磷酸盐法提取杏仁蛋白,对蛋白没有破坏,提取率较高,为其进一步开发奠定了基础。

Abstract

Objective To extract almond protein by phosphate method from among dregs,and confirm the best process conditions. Methods Based on designing and analyzing single factor experiment and orthogonal experiment,the dregs and phosphate solution were mixed, heated in water bath and stirred, centrifugally separated, absorbance of liquid supernatant was measured, and extraction rate was calculated to confirm process parameter. Results The process conditions of extracting almond protein by phosphate method is temperature 50℃, feed liquid ratio 1:30,time 1.0 h, salting strength 0.01mol/L,and protein extract rate is 72%. Conclusions Extraction of almond protein by phosphate method will not destroy protein and is high in extraction rate.

关键词

杏仁蛋白 / 磷酸盐 / 提取工艺 / 正交实验

Key words

almond protein / phosphate / extract process conditions / orthogonal experiment

引用本文

导出引用
刘凯,梁俊玉,肖引,陈江,陈芳. 磷酸盐法提取杏仁蛋白的工艺研究[J]. 武警医学. 2013, 24(9): 783-785
LIU Kai,LIANG Junyu,XIAO Yin,CHEN Jiang,and CHEN Fang .. Extracting process conditions from almond dregs by phosphate method[J]. Medical Journal of the Chinese People Armed Police Forces. 2013, 24(9): 783-785
中图分类号: TS255.6   

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