目的 探讨PTEN及survivin基因在视网膜母细胞瘤组织中的表达及临床意义。方法 选择30例视网膜母细胞瘤组织为观察组, 30例正常视网膜组织为对照组, 应用免疫组织化学方法检测两组切片中PTEN、survivin基因的表达, 比较两组PTEN和survivin表达差异。结果 观察组PTEN总阳性表达率为53.3%, 对照组PTEN总阳性表达率为100.0%, 两组差异有统计学意义(P<0.05);观察组survivin总阳性表达率为60.0%, 对照组survivin总阳性表达率为0.0%, 两组差异有统计学意义(P<0.05)。结论 PTEN基因阳性表达降低与survivin基因阳性表达升高, 是视网膜母细胞瘤的发病机制之一。

Objective To study the expressions and clinical significance of PTEN and survivin in retinoblastoma tissues. Methods Retinoblastoma tissue sections from 30 patients were selected as observation group, 30 healthy retinal tissue sections were selected as control group. Immunohistochemical method was used to detect the PTEN and survivin expressions, Expressions of PTEN and survivin were compared between the two groups, and their clinical significance was discussed. Results In the observation group, PTEN-positive expression rate was 53.3%;in the control group, the total positive expression rate of PTEN was 100%. In the observation group, the total positive expression rate of survivin was 60%;in the control group, the total positive expression rate of survivin was 0%;the difference between the two groups was significant(P<0.05). Conclusions In retinoblastoma tissues, the survivin-positive expression was significantly increased and PTEN-positive expression was significantly reduced.