目的 探讨慢性丙型肝炎患者树突状细胞(dendritic cells, DC)体外培养后细胞因子IL-12、INF-γ水平变化。方法 选择32例慢性丙型肝炎患者作为观察组, 30例健康体检者作为对照组, 分别取抗凝血50 ml, 经过DC体外培养后留取上清液5 ml备检, 用ELISA试剂盒检测两组培养前、后细胞因子IL-12、INF-γ的水平变化。结果 观察组实验前血清中细胞因子IL-12为(38.46±4.97)ng/L, INF-γ为(15.27±2.67)pg/ml, 对照组分别为(61.10±4.38)ng/L、(27.24±1.79)pg/ml , 两组差异有统计学意义(P＜0.01)。观察组DC培养后上清液中IL-12为(337.91±13.35)ng/L, INF-γ水平为(6076.72±403.43)pg/ml, 对照组分别为(661.44±52.99)ng/L、(10 449.00±626.54)pg/ml , 两组差异有统计学意义(P＜0.01)。结论 体外培养后DC分泌IL-12、INF-γ水平明显高于培养前, 提示通过DC培养提高细胞因子水平诱导细胞免疫, 可作为慢性丙型肝炎的一种新型辅助疗法。

Objective To study the changes in levels of cytokines IL-12 and INF-gamma in patients with chronic hepatitis C after culture in vitro of dendritic cell (DC). Methods Thirty-two patients with chronic hepatitis C were recruited as the observation group, and 30 healthy subjects undergoing physical examination as the control group, 50 ml of anticlotting blood was taken separately. After culture of DC in vitro, 5 ml of supernatant fluid were returned for examination. Using ELISA kit, the cytokines IL-12, INF-gamma level changes before and after culture in the two groups were examined. Results In the observation group, before the experiment, serum cytokine IL-12 was (38.46?4.97) ng/L and IFN - gamma was (15.27?2.67) pg/ml; in the control group, the cytokines were (61.10?4.38) ng/L and (27.24?27.24) pg/ml, respectively; the difference between the two groups was statistically significant (P<0.01). In the observation group, after culture of DC, IL-12 in the supernatant fluid was (337.91?13.35) ng/L, IFN-gamma was (6076.72?403.43) pg/ml; in the control group, the cytokines were (661.44?52.99) ng/L and (10 449.00?626.54) pg/ml, respectively; the difference between the two groups was statistically significant (P<0.01). Conclusions After in vitro culture of DC, secretion of IL-12, and the INF-gamma is obviously higher than that before culture, suggesting that raising the level of cytokines induced by DC immune cells can be used as a new auxiliary therapy in the treatment of chronic hepatitis C.