目的 对P63蛋白在正常肺脏及肺癌组织中的分布进行定量分析,判断其表达与细胞凋亡的关系。方法 收集肺癌组织标本及肿物周围正常肺组织,制备组织芯片。以免疫组织化学方法鉴定P63表达并进行量化分析;以脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法判断细胞凋亡并进行量化分析;最终将结果量化为阳性单位(PU)进行分析。结果 肺鳞癌细胞核P63的PU值大于正常肺支气管上皮、肺腺癌、大细胞癌、小细胞癌细胞核P63的PU值(P＜0.01);肺腺癌、大细胞癌、小细胞癌的PU值小于正常肺支气管上皮细胞核P63的PU值(P＜0.01);正常肺组织中P63的表达与细胞凋亡之间无相关关系(r＝0.1619,P＞0.05);肺癌组织中P63的表达与细胞凋亡呈正相关(r＝0.3333,P＜0.001)。结论 正常肺组织中P63的表达量和细胞凋亡是两个独立存在的事件;而肺癌组织中P63的表达与细胞凋亡呈正相关。
Abstract
Objective To analyze the expression of protein p63 in normal lung and lung cancers quantitatively and judge its relationship with cellular apoptosis. Methods Lung cancer and normal lung tissues were collected and tissue arrays were prepared. The p63 expression was determined by immunohistochemistry and analyzed quantitatively. The cellular apoptosis was analyzed quantitatively by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The final qualified results (positive unit, PU) were analyzed statistically. Results The PU values of the intensity of p63 protein expression in normal lung tissue, adenocarcinoma, large cell carcinoma and small cell carcinoma were all lower than that in squamous carcinoma (P＜0.01); The PU value of normal lung tissue was higher than those in adenocarcinoma, large cell carcinoma and small cell carcinoma (P＜0.01); while there was a positive correlation between P63 protein expression and apoptosis in all kinds of lung cancer (P＜0.01). Conclusions The extents of protein p63 expression are distinct on the normal lung and lung carcinoma tissues. The p63 expression and cellular apoptosis on the normal lung tissues are separated. However, p63 expression and cellular apoptosis on the lung malignant tissues are positively correlated.
关键词
P63 /
肺癌 /
凋亡 /
定量分析
Key words
p63 /
lung cancer /
apoptosis /
quantitative analysis
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Joerger A C, Rajagopalan S, Natan E, et al. Structural evolution of P53, P63, and p73: implication for heterotetramer formation[J]. Proc Natl Acad Sci U S A, 2009, 106(42):17705-17710.
[2] Thomason H A, Zhou H, Kouwenhoven E N, et al.Cooperation between the transcription factors P63 and IRF6 is essential to prevent cleft palate in mice[J].J Clin Invest, 2010,120(5):1561-1569.
[3] Huang W,Liu K.P53 family proteins provide new insights into lung carcinogenesis and clinical treatment [J].Zhongguo Fei Ai Za Zhi, 2013,16(8):422-426.
[4] Kim D H, Kwon M S. Role of fine needle aspiration cytology, cell block preparation and CD63, P63 and CD56 immunostaining in classifying the specific tumor type of the lung[J].Acta Cytol, 2010, 54(1):55-59.
[5] Shimada Y, Ishii G, Nagai K, et al. Expression of podoplanin, CD44, and P63 in squamous cell carcinoma of the lung[J]. Cancer Sci, 2009,100(11):2054-2059.
[6] NapsinA.TTF-1、CK5/6、CK7、P63在非小细胞肺癌支气管镜活检标本中的表达及意义[J].肿瘤防治研究,2013,40(1):76-78.
[7] Yao J Y,Chen J K. Roles of P63 in epidermal development and tumorigenesis[J]. Biomed J,2012,35(6):457-463.
[8] Glickman J N, Yang A, Shahsafaei A, et al.Expression of P53-related protein P63 in the gastrointestinal tract and in esophageal metaplastic and neoplastic disorders[J]. Hum Pathol, 2001,32(11):1157-65.
[9] Massion P P, Taflan P M, Jamshedur S M, et al. Significance of P63 amplification and overexpression in lung cancer development and prognosis[J].Cancer Res, 2003, 63(21):7113-7121.
[10] 梁喜林,孟 晓,赵 红.前列腺良恶性病变中P504S、P63、CK34βE12表达的研究[J].武警医学,2010,21(3):200-201.
[11] 刘换新,王彩霞,刘 梅.宫颈毛玻璃细胞癌临床病理观察[J].武警医学,2013,24(2):139-142.
[12] 万蔚平, 赵妍妍, 吴开松.P63和CK5/6在肺癌组织中的表达及临床意义[J].中华临床医师杂志, 2014,8(3):444-447.
[13] Jacobs J J, Lehe C , Cammans K D, et al. An automated method for the quantification of immunostained human Langerhans cells[J]. J Immunol Method, 2001,247(1-2):73.
[14] Huang F,Shen H.Inter random number generator-based true random number generator[J].Di Yi Jun Yi Da Xue Xue Bao,2004,24(9):1091-1095.
[15] 韩 伟.TGF-β1和PTEN在非小细胞肺癌组织中的表达及相关关系定量分析[J].中国体视学与图像分析,2014,19(1):63-69.
[16] Pavlovic V, Sarac M. The role of ascorbic acid and monosodium glutamate in thymocyte apoptosis[J].Bratisl Lek Listy, 2010,111(6):357-360.
PDF(540 KB)
