目的 探讨白细胞介素-6(IL-6 gene ,IL-6)基因启动子区-634C/G多态性与中国广东地区汉族人糖尿病(diabetes mellitus,DM)患者视网膜病变(diabetic retinopathy ,DR)的关系。方法 在中国广东地区汉族人中,应用聚合酶链反应限制性片段长度多态性(PCR-RFLP )方法,对245例2型糖尿病(T2DM)患者,其中正常眼底即DR0组153例,非增殖期视网膜病变即DR1组51例,增殖期视网膜病变即DR2组41例,和101例健康对照者(NC组)的IL-6基因启动子区-634C/G多态性进行了检测,并比较分析各组间基因型频率和等位基因频率以及相关临床资料。结果 (1)DR0、 DR1、 DR2三组间在年龄、性别、血压、体重指数、腰臀比、空腹血糖、餐后2 h血糖、糖化血红蛋白A1c、血脂等指标均无统计学差异,而病程在三组间差异具有统计学意义(F=19.05,P＜0.05)。(2) 基因型GG在NC、DR0、DR1、DR2各组间差异有统计学意义(χ2=20.839,P=0.002),DR1、DR2组GG基因型明显高于DR0组,差异有统计学意义(χ2=7.615,P=0.022);等位基因G在NC、DR0、DR1、DR2各组间差异有统计学意义(χ2=11.687,P=0.009),但两组间比较差异无统计学意义。(3)Logistic回归分析表明,IL-6基因启动子区-634G/G 基因型、病程及空腹血糖是DR发生的危险因素。结论 在中国广东地区汉族人中,IL-6基因启动子区-634 G/G基因型是DR的遗传危险因素。
Abstract
Objective To investigate the association of IL-6 gene promoter region -643C/G polymorphism with diabetic retinopathy. Methods The polymorphism of IL-6 gene promoter region -634 C/G in 245 unrelated Guangdong Han people with type 2 diabetes ,including 153 cases without diabetic retinopathy (DR0 group) ,51 cases without proliferative diabetic retinopathy (NPDR)(DR1 group), 41 cases with proliferative diabetic retinopathy (PDR) (DR2 group), and 101 normal controls(NC group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. The genotype frequency, allele frequency and relative clinical data were compared between groups. Results (1)There were no significant differences in the clinical characteristics except for diabetic duration among DR0, DR1, DR2 groups. (2) The frequencies of IL-6-643 G/G genotype and G allele frequencies among DR0 DR1 DR2 groups were statistically different. There were significantly different in GG genotype between DR0 and DR group,comparison between any two means were not significant differences in G allele frequencies.(3) Logistic regression analysis showed that IL-6 gene promoter region-643G/G genotype,fasting plasma glucose and diabetic duration were risk factors of DR. Conclusions The IL-6 promoter region-643G/G genotype may be a genetic risk factor of DR development.
关键词
白细胞介素-6基因 /
启动子区 /
多态性 /
糖尿病视网膜病变
Key words
IL-6 gene /
promoter region /
polymorphism /
diabetic retinopathy
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参考文献
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基金
广东省中山市科技局基金资助项目(2006A035)
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