目的 分析周围型肺腺癌在CT上的一些典型征象,并探讨这些征象与CD147、VEGF表达的关系。方法 收集2006-01至2012-12周围型肺腺癌73例可作免疫组化的蜡块及术前CT检查资料。采用免疫组化SP法分别测定肺腺癌组织中CD147及VEGF的表达结果,对阳性结果根据染色面积及染色强度进行等级划分。将CD147、VEGF的表达结果与术前CT检查的各特异性征象进行对比,观察两种基因表达结果与CT征象之间是否相关。结果 CD147表达与CT征象中的空泡征、肿块大小、棘突征、深分叶征、纵隔淋巴结转移之间有相关性(P＜0.05),与毛刺征、胸膜凹陷征无相关性(P>0.05);VEGF表达与毛刺征、棘突征、深分叶征、纵隔淋巴结转移之间有相关性(P＜0.05),与肿块大小、空泡征、胸膜凹陷征无相关性(P>0.05)。结论 肺腺癌的某些CT特异性征象与CD147、VEGF表达有相关性,可为判断肺腺癌的预后提供依据。
Abstract
Objective To analyze the pathological mechanism of the typical features of lung adenocarcinoma in CT imaging,and to study the relationship between the features and expression levels of CD147 and VEGF. Methods In China-Japan Friendship Hospital and Air Force general Hospital, from 2006 to 2012, 73 cases of lung adenocarcinoma were pathologically confirmed. The wax block for immunohistochemistry and preoperative CT scan data were collected. Using immunohistochemical SP to detect the expression results of CD147 and VEGF in lung adenocarcinoma, positive results were graded according to size and staining intensity of staining. CT signs included spiculation, vacuole sign, spinal, tumor size, deep lobulation, pleural retraction and mediastinal lymph node metastasis. To compare and determine the relationship between the expressions of CD147and VEGF and the features of lung adenocarcinoma CT imaging, and to observe whether there existed correlation between the expression of two genes results and CT signs. Results There was a correlation between the expression of CD147 and CT signs of the syndrome in vacuoles,tumor size,spiculation sign,deep lobulation,mediastinal lymph node metastasis(P＜0.05),no correlation with spiculation and pleural indentation(P>0.05).There was correlation between the expression of VEGF and spiculation,spinal,deep lobulation, mediastinal lymph node metastasis (P＜0.05),no correlation with tumor size,vacuole sign,pleural indentation sign (P>0.05). Conclusions There is a correlation between some specific CT signs of adenocarcinoma of lung and the expression rate of CD147 and VEGF. The research on the relationship of CD147/VEGF expression and CT signs is helpful to further explore the development process of the tumor, more accurately judge the prognosis of lung adenocarcinoma.
关键词
肺腺癌 /
CT /
免疫组织化学 /
血管内皮生长因子 /
细胞外基质金属蛋白酶诱导因子
Key words
adenocarcinoma /
computed tomography /
immunohistochemistry /
CD147 /
vascular endothelial growth factor
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