目的 观察高压氧干预(hyperbaric oxygenation treatment, HBOT)对大鼠创伤性脑损伤(traumatic brain injury,TBI)后NF-κB(p50) 表达的影响,并探讨其机制。方法 将35只SD大鼠完全随机分为假手术对照组(Con组)5只、创伤组(TBI)15只、高压氧干预组(HBOT)15只。采用Allen’s改良法制造大鼠自由落体重型脑损伤模型,Con组仅切开头皮去骨窗,TBI组给予撞击损伤,HBOT组于创伤后给予高压氧治疗。利用Western-blot法分别于伤后8 h及1、3、5、8 d检测脑组织NF-κB的表达。结果 与Con组比,TBI组各时点脑组织NF-κB表达均升高(P＜0.05),损伤后3 d达高峰(0.7267±0.0305),5～8 d仍维持较高水平(0.5567±0.0603)。HBOT组各时点NF-κB 表达水平均较TBI组下降(P＜0.05)。结论 高压氧干预可以减弱创伤后脑组织NF-κB(p50)表达,有效抑制炎性反应,为重型颅脑损伤治疗提供理论基础。
Abstract
Objective To observe the effect of hyperbaric oxygenation treatment(HBOT) on expression of NF-κB(p50) in the rat brain tissue after traumatic brain injury(TBI) and discuss its mechanism. Methods Thirty-five Sprague-Dawley rats were divided into sham operation control group(5 rats), TBI group(15 rats) and HBOT group(15 rats). Based on the Feeney’s model of modified Allen’s method, experimental animals were treated with HBOT. The animals were sacrificed at the 8th hour, and on days 1,3,5,8,respectively. The expression of protein NF-κB was determined by Westernblotting. Results The expression of NF-κB was significantly higher in traumatic group than that in control group at the time of 8 hour and 1,3,5and 8 days after injury (P＜0.05).The level of NF-κB reached peak on the 3th day, and maintained a high status from 5 to 8 days. Compared with traumatic group, the NF-κB level was significantly lower in the HBOT group (P＜0.05). Conclusion HBOT can weaken NF-κB expression in traumatic brain tissue and inhibit effectively inflammatory response, which provides theoretical basis for treating severe traumatic brain injury.
关键词
重型颅脑损伤 /
高压氧 /
大鼠 /
NF-κB
Key words
severe brain injury /
hyperbaric oxygenation /
rats /
NF-κB
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