目的 探讨泪腺腺样囊性癌S-100蛋白及神经细丝酸性蛋白(glial fibrillary acidic protein, GFAP)的表达及其意义。方法 收集手术切除泪腺腺样囊性癌28例标本,根据临床症状及组织学观察分为嗜神经侵袭组(15例)和无嗜神经侵袭组(13例),并取术中切除的正常泪腺组织9例为正常对照。应用免疫组织化学SP染色法检测S-100及GFAP在上述组织中表达的差异。结果 嗜神经侵袭性生长组的S-100、GFAP阳性表达分别为12例(80%)和9例(60%),与无嗜神经侵袭性生长组S-100阳性5例(41.67%)、GFAP 阳性表达率为0相比,呈显著升高趋势,差异有统计学意义 (P＜0.05)。结论 GFAP、S-100在有嗜神经性侵袭的腺样囊性癌中有特异性表达,其侵袭特性可能与肿瘤细胞中存在着神经膜细胞分化有关。
Abstract
Objective To investigate the expression of Schwann's cell markers S-100 and GFAP in lacrimal adenoid cystic carcinoma, and to explore the relationship between GFAP or S-100 expression and neural invasion of adenoid cystic carcinoma. Methods Twenty-eight lacrimal adenoid cystic carcinoma and 9 normal lacrimal gland specimens were collected . Each specimen was stained by S-P(streptavidin/peroxidase) immuno- histochemical staining. The expression of S-100 and GFAP was analyzed by immunohistochemistry in order to find the difference in the above-mentioned tissues. One-way ANOVA, Student,s test, chi-square test or linear correlation were carried out for statistical evaluation. Results The average S-100 Index(S-100LI) in nor-neural invasion group and neural invasion group were 0.62±1.19 and 16.73±24.74,respectively,in lacrimal gland adenoid cystic carcinoma. (P<0.05). The average GFAP LI in nor-neural invasion group and neural invasion group were 0 and 2.07±3.01,respectively,in lacrimal gland adenoid cystic carcinoma. (P<0.05). Conclusions Schwann’s cell markers GFAP and S-100 in neural invasion group is higher than that in nor-neural invasion group (P<0.05). The Schwann’s cell differentiation may account histologically for the neural invasion of lacrimal gland adenoid cystic carcinoma.
关键词
泪腺腺样囊性癌 /
S-100 /
神经细丝酸性蛋白 /
嗜神经性 /
免疫组织化学
Key words
lacrimal adenoid cystic carcinoma /
S-100 /
glial fibrillary acidic protein /
neural invasion /
immunohistochemistry
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