目的研究重楼皂苷对成骨样细胞MC3T3-E1细胞增殖的作用及其机制。方法 培养成骨样细胞MC3T3-E1, 加入不同浓度的重楼皂苷(3、10、30、100 μg/ml), 采用MTT比色试验法、EdU试剂盒、碱性磷酸酶(ALP)活性检测法观察MC3T3-E1的增殖情况, 以Western blot方法观察重楼皂苷 (30、100 μg/ml)对成骨样细胞中β-连环素(β-catenin)和骨形成蛋白-2 (BMP-2)表达的影响。结果 重楼皂苷可有效促进成骨样细胞MC3T3-E1的增殖(增殖率117.67～153.33), 浓度依赖性地升高β-catenin与BMP-2的表达。结论 重楼皂苷可能通过调节β-catenin与BMP-2信号通路促进MC3T3-E1细胞增殖与分化。
Abstract
Objective To investigate the effect of polyphyllin on the proliferation of osteoblast-like cells MC3T3-E1 and possible mechanisms. Methods The osteoblast-like cells MC3T3-E1, were administered with different concentrations of polyphyllin (3, 10, 30, and 100 μg/ml). Then, MTT colorimetric test, EdU kit, and alkaline phosphatase (ALP) activity detection kit were used to observe the proliferation of MC3T3-E1. Western blot analysis was employed to observe the expression of β-catenin and bone morphogenetic protein -2 (BMP-2) in MC3T3-E1 cells treated with 30, and 100 μg/ml of polyphyllin. Results Polyphyllin could effectively promote proliferation of MC3T3-E1 cells and upregulate the expression of β-catenin and BMP-2 expression. Conclusions Polyphyllin may promote MC3T3-E1 cell proliferation and differentiation via regulating β-catenin and BMP-2 signaling pathway.
关键词
重楼皂苷 /
成骨样细胞MC3T3-E1 /
增殖 /
骨形态发生蛋白2 /
β-连环素
Key words
polyphyllin /
osteoblast-like cells MC3T3-E1 /
proliferation /
bone morphogenetic protein 2 /
β-catenin
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参考文献
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