目的 探讨miR21抑制剂转染人肺腺癌细胞A-549对其体外侵袭及血管形成能力的影响。方法 miR21抑制剂转染A-549细胞,同时设置未转染的A-549细胞作为对照组,转染24、48、72及96 h后,四甲基偶唑氮法(MTT)法检测两组细胞增殖抑制率;Transwell方法检测miR21抑制剂对A-549细胞侵袭的影响;小管形成实验检测miR21抑制剂对血管形成的影响。结果 转染24 h,两组抑制率比较,差别无统计学意义;转染48 h及以后,抑制剂组抑制率均高于对照组,差别有统计学意义(P<0.05)。Transwell检测结果显示miR21抑制剂组每个视野细胞数(11.60±5.32)个远少于对照组(107.60±3.21)个,两组差别有统计学意义(P<0.01)。抑制剂组每视野小管计数平均(159.30±0.51)个,远少于对照组(508.80±1.30)个,两组差别有统计学意义(P<0.01)。结论 miR21抑制剂能够明显抑制A-549的侵袭能力,并能明显抑制血管内皮细胞血管生成,提示miR21抑制剂可以作为潜在的肺癌基因治疗的新方法。

Objective To study the inhibition of miR21 inhibitor on the invasion and anti-angiogenesis of A-549 cells. Methods MiR21 inhibitor was transfected into A-549, and the control group was set up. 24, 48, 72, 96 hours after transfection, the effect of growth suppressing was quantified by 3-2(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide(MTT) assay, anti-invasion was observed by transwell assay. Tube formation assay was used to detect the effects of miR21 inhibitor on human umbilical vein endothelial cells (HUVEC) angiogenesis. Results Twenty-four hours after transfection, the suppressing rate was different between the two groups; 48, 72, 96 h after transfection, the suppressing rates in inhibition group were higher than those in control group, the difference was statistically significant(P<0.05). By transwell assay, number of cells in inhibition group (11.60?5.32) was less than that in control group (107.60?3.21), the difference was statistically significant(P<0.01). Tubes in inhibition group (159.30?0.51) was less than that in control group (508.80?1.30), the difference was statistically significant(P<0.01). Conclusions miR21 inhibitor can inhibit the invasion of A-549 and angiogenesis of HUVEC. miR21 inhibitor can be a potential gene-therapy for gastric carcinoma.