目的 探讨军事演习前后部队官兵血清和胃黏膜肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平及其意义。方法 分别采用酶联免疫和免疫组化的方法检测某部60名官兵军事演习(简称军演)前后血清和胃黏膜TNF-α的水平。结果 军演前官兵外周血血清中TNF-α的含量为(38.05±15.49) pg/ml,军演后为(92.93±30.98) pg/ml,较军演前明显上升(P＜0.01)。军演前后TNF-α均主要表达于胃黏膜固有层腺上皮细胞和间质单个核细胞中,军演后该分子的表达明显强于军演前(P＜0.01)。结论 TNF-α可能中介了军事应激所致的胃黏膜病变。在军事应激早期检测该细胞因子的含量,有助于评估发生胃黏膜病变的可能性及严重程度。

Objective To investigate the levels of TNF-α in serum and gastric mucosa before and after military stress and their significances. Methods The level of serum TNF-α in 60 soldiers was studied by enzyme-linked immunosorbent assay. The expression of TNF-α in gastric mucosa from the 60 soldiers was detected by immunochemical staining. Results The level of TNF-α in serum in the soldiers was (92.93±30.98)pg/ml after military maneuver, and was significantly higher than that of (38.05±15.49) pg/ml before military maneuver (P<0.01). TNF-α was found to be expressed in the lamina propria epithelioglandular cells and in the mesenchymal mononuclear cells in gastric mucosa of the soldiers before and after military maneuver. The expression of TNF-α was also significantly stronger after military maneuver compared with that before military maneuver (P<0.01). Conclusions TNF-α might be involved in the development of the pathological changes of gastric mucosa caused by military stress in the troops. It will be helpful to examine the level of TNF-α in the early stage of military stress for evaluating the possibility of the occurrence of gastric mucosa lesion and its severity.