目的探索肝脏移植并FK506联合用药后人血清中蛋白质组的变化,研究免疫排斥反应的机制及FK506联合用药的作用。方法取5例肝癌患者移植前和移植后24 h、10 d、30 d、90 d、180 d的血清样品,用Aurum Serum Protein Mini Kit除高峰度蛋白,进行双向电泳(2-DE)分离,ImageMaster 2D Platinum图像分析软件分析电泳图谱。结果建立移植前和移植后并FK506联合用药的人血清双向电泳差异图谱,蛋白斑点数在(877±59)~(897±61),移植前、后不同组之间的匹配均>70%。共筛选出重复性好且表达显著差异的蛋白质斑点7个。结论初步建立了肝移植并FK506联合用药后人血清蛋白质组的2-DE分析方法。

Objective To analyze the serum proteome change after liver transplantation and FK506 combination in human serum by two-dimensional gel electrophoresis (2-DE) and to study the mechanism of immune rejection and the mechanism of action of FK506 combination. Methods The serum samples of 5 liver transplantation patients pre- and post transplantation at 24 hours,10 days,30 days,90 days 180 days were treated using Aurum Serum Protein Mini Kit, and than analyzed by 2-DE. The electrophoresis images were analyzed by using ImageMaster 2D platirum imaging analysis software. Results The differential maps of 2-DE of serum proteome before and after liver transplantation and FK506 combination were established. Protein spots between (877?59.65)-(897.6?61.01),the matching rate before transplantation and different groups after transplantation>70%. Among them 7 protein spots were selected with good repeatability and significant differential expression. Conclusions The 2-DE analytical method of serum proteome after liver transplantation and FK506 combination was preliminarily established. The differential expression protein spots found in the study have laid a foundation for the further study .