目的研究依达拉奉对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis, EAE)小鼠的抑制作用。方法将60只雌性C57BL/6小鼠随机分为3组:正常对照组、EAE组、EAE+依达拉奉组,每组20只。正常对照组用PBS作为对照。后两组应用髓鞘少突胶质细胞糖蛋白35-55、完全福氏佐剂(CFA)、结核分枝杆菌,制成的抗原乳剂进行造模。免疫2 d后,EAE+依达拉奉组腹腔注射依达拉奉5 mg/(kg·d)。3组均监测20 d,每日称重,观察小鼠的摄食和发病情况,并进行神经功能评分。20 d后取小鼠脊髓组织,进行病理学观察。结果EAE组小鼠的发病率(80%)高于EAE+依达拉奉组(45%),差异有统计学意义(P＜0.05)。EAE+依达拉奉组神经功能评分各个时间点都低于EAE组,差异有统计学意义(P＜0.05),其中,监测10 d后,EAE组神经功能评分(3.6±0.4)级;而EAE+依达拉奉组评分为(1.3±0.5)级。HE染色后发现,EAE组小鼠的脊髓内有大量的炎性细胞浸润,白质脱髓鞘改变明显。而EAE+依达拉奉组小鼠有较少的炎性细胞浸润,且没有白质脱髓鞘改变。结论依达拉奉对EAE的保护作用可能与其清除自由基、减轻炎性反应有关。

Objective To study the inhibiting effect of edaravone on experimental autoimmune encephalomyelitis (EAE) mouse model. Methods Sixty female C57BL / 6 mice were randomly divided into three groups: normal control group, EAE group, EAE + edaravone group (n=20), normal control group with PBS as a control. The application of myelin oligodendrocytes glial cells glycoprotein 35-55 and complete Freund’s adjuvant (CFA) and Mycobacterium tuberculosis antigens emulsions were made modeling in the later two groups. After 2 days immunization, EAE+edaravone group were injected at a dose of 5 mg/(kg·d) of edaravone. Three groups were monitored 20 days, daily weighing, feeding and incidence were observed, and neurological function was scored. Mouse spinal cord tissue was obtained for pathological examination after 20 days. Results In EAE+edaravone treated mice, compared with EAE mice, the incidence and neurological function scores were significantly low (P<0.05). The incidence in EAE group was 80%, while the incidence in EAE+ edaravone group mice was 45%. Monitored for 10 days, in EAE group mice, neural function score was 3.6±0.4, while in EAE+ edaravone group mice neural function score was 1.3±0.5. HE staining found a large number of inflammatory cell infiltration in EAE mice spinal cord, and white matter demyelination was obvious. Nevertheless edaravone group mice had fewer inflammatory cell infiltration, and no white matter demyelination. Conclusions Protective effect of edaravone on EAE is related to scavenging free radicals and reducing inflammation.