目的 探讨CML28与WT1分子在急性白血病(acute leukemia, AL)患者原代骨髓细胞中的表达及表达相关性。方法 收集华中科技大学附属同济医院2009-01至2009-06收住入院的29例初治AL患者骨髓单个核细胞,采用Trizol一步法提取细胞总RNA,逆转录反应合成cDNA,通过反转录PCR (reverse transcription PCR,RT-PCR)及实时定量PCR(Realtime PCR)检测CML28与WT1分子的mRNA表达情况,并通过相对定量公式2-ΔΔCT计算CML28及WT1分子的mRNA相对表达水平。结果 29例初治AL患者骨髓单个核细胞同时高表达CML28及WT1分子,二者表达水平呈正相关关系(r=0.462,P=0.008)。结论 CML28及WT1分子同时高表达于各种急性白血病,且呈明显的正相关关系。
Abstract
Objective To study the expression level and correlation between CML28 and WT1 in acute leukemia(AL) patients’ bone marrow( BM) primary cells.Methods Bone marrow mononuclear cells (MMNC) were collected from 29 AL patients who were initially treated and admitted to the Tongji Hospital affiliated to Huazhong University of Science＆Technology from January to June in 2009.Total mRNA was extracted from MMNC of AL patients using Trizol reagents, and the first cDNA was synthesized using reverse transcription kits. Their mRNA expression levels were detected in AL patients by RT-PCR and real time PCR and their mRNA expression levels were quantitated by relevant quantitative formula 2-ΔΔCT.Then the correlation coefficient was calculated using SPSS 17.0 statistical software. Results There was a high expression level of CML28 and WT1 in the MMNC of AL patients at the same time, with significant difference between their mRNA expression leuels (r=0.462,P=0.008). Conclusions CML28 and WT1 are highly expressed in a variety of acute leukemia, and they have an obviously positive correlation.
关键词
CML28 /
WT1 /
急性白血病 /
相关性
Key words
CML28 /
WT1 /
acute leukemia /
correlation
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Renneville A, Roumier C, Biggio V, et al.Cooperating gene mutations in acute myeloid leukemia:a review of the literature[J]. Leukemia, 2008,22(5):915-931.
[2] Scholl C,Gilliland D G,Frhling S.Deregulation of signaling pathways in acute myeloid leukemia[J]. Semin Oncol,2008,35(4):336-345.
[3] Yang X F,Wu C J, Chen L,et al. CML28 is a broadly immunogenic antigen, which is overexpressed in tumor cells [J].Cncer Res, 2002,62(19):5517-5522.
[4] Zhou H, Zhang D,Wang Y, et al. Induction of CML28-specific cytotoxic T cell responses using co-transfected dendritic cells with CML28 DNA vaccine and SOCS1 small interfering RNA expression vector [J]. Biochem Biophys Res Commun,2006, 347(1):200-207.
[5] Zhang D H,Zhou H S,Y Y,et al.Construction and expression of dendritic cell nucleic acid vaccine containing CML28 gene in human dendritic cells [J].Zhongguo Shi Yan Xue Ye Xue Za Zhi,2005,13(4):631-636.
[6] 张东华,戴 敏,周红升,等. 实时定量RT-PCR监测造血干细胞移植前后CML28 mRNA表达[J].中国实验血液学杂志,2005,13(5):843-847.
[7] Inoue K,Sugiyama H,Ogawa H,et al.WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia[J].Blood,1994,84(9):3071-3079.
[8] Catherine J W,Melinda B,Jeffery L, et al. Graft-versus-Leukemia Target Antigens in Chronic Myelogenous Leukemia Are Expressed on Myeloid Progenitor Cells[J].Clin Cancer Res,2005,11(12):4504-4511.
[9] Liu Q,Greimann J C. Reconstitution,Activities,and Structure of the Eukaryotic RNA Exosome [J].Cell,2006,127(6),1223-1237.
[10] Fasken M B,Corbett A H.Mechanisms of nuclear mRNA quality control[J].RNA Biol, 2009, 6(3):237-241.
[11] Ladomery M,Dellaire G.Multifunctional zinc finger proteins in development and disease[J].Ann Um Genet,2002,66(Pt 5-6):331-342.
[12] Davies J A,Ladomery M,Hohenstein P,et al.Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation[J].Hum Mol Genet,2004,13(2):235-246.
[13] Ariyaratana S, Loeb D M. The role of the Wilms tumour gene (WT1) in normal and malignant haematopoiesis[J]. Expert Rev Mol Med, 2007, 9(14):1-17.
[14] Bergmann L, Miething C, Maurer U, et al. High levels of Wilms’ tumor gene (wt1) mRNA in acute myeloid leukemias are associated with a worse long-term outcome[J]. Blood, 1997, 90(3): 1217-1225.
[15] Guo X,Ma J,Sun J,et al.The zinc-finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA [J].Proc Natl Acad Sci U S A, 2007,104(1):151-156.
PDF(740 KB)
