基质辅助激光解吸电离飞行时间质谱在临床微生物检测和鉴定中的应用

宗玉龙,曹源,褚芳波,张萍萍综述胡成进审校

武警医学 ›› 2014, Vol. 25 ›› Issue (9) : 958-960.

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PDF(495 KB)
武警医学 ›› 2014, Vol. 25 ›› Issue (9) : 958-960.
综述

基质辅助激光解吸电离飞行时间质谱在临床微生物检测和鉴定中的应用

  • 宗玉龙1,曹源2,褚芳波1,张萍萍1综述胡成进2审校
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摘要

现今临床主要通过观察细菌菌落形态、革兰染色、显微镜检查,以及各种生化试验等方法对致病菌进行检测和鉴定。这些方法主要依赖致病菌的生长代谢,其周期较长,并且带有一定的主观性[1],不能满足临床上对致病菌进行快速诊断的需要。基质辅助激光解吸电离飞行时间质谱(matrix assisted laser desorption/ionization time-of-fight mass spectrometry,MALDI-TOF MS)的诞生和发展奠定了用蛋白质指纹图谱鉴定微生物的基础。通过检测未知微生物的蛋白质指纹图谱并与质谱图数据库中特征性谱图进行比对,从而对待测微生物进行快速鉴定。该技术具有速度快,灵敏度高,操作简单,信息直观,成本低廉等特点,在对病原微生物进行快速鉴定方面具有良好的应用前景。

关键词

基质辅助激光解吸电离飞行时间质谱 / 微生物 / 检测 / 鉴定

引用本文

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宗玉龙,曹源,褚芳波,张萍萍综述胡成进审校. 基质辅助激光解吸电离飞行时间质谱在临床微生物检测和鉴定中的应用[J]. 武警医学. 2014, 25(9): 958-960
中图分类号: R446.5   

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基金

全军十二五重点基金资助项目 (BWS12J014)

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