目的 探讨钙调蛋白(calmodulin, CaM)、钙/钙调蛋白依赖性激酶Ⅱ(Ca2+/calmodulin kinaseⅡ, CaMKⅡ)在大鼠海马发育中的变化规律。方法 以出生后第1、7、14、21、30和 50天的大鼠为研究对象, 采用实时定量PCR和Western blot方法分别检测海马CaM和CaMKⅡ的mRNA及蛋白的表达。结果 大鼠海马CaM及CaMKⅡ在出生后第1天时表达较弱, 第14～30天均快速增加(P＜0.05或P＜0.01), 呈增量表达;第50天后表达持续在高水平(CaMmRNA和CaMKⅡmRNA相对值分别为2.242±0.154和2.418±0.329;CaM蛋白和CaMKⅡ蛋白相对值分别为0.782±0.061和2.194±0.256;P＜0.05或P＜0.01), 但较出生后第21天已有减弱趋势。结论 CaM和CaMKⅡ在海马中的表达变化具有一定的时序性, 此模式可能与突触的发育及脑成熟变化密切相关, 也与学习记忆功能的形成密切相关。
Abstract
Objective To study the changes in expressions of CaM、CaMKⅡmRNA and protein in the hippocampus of rat during postnatal development. Methods Real-time PCR and Western blot were used to detect the different expressions of mRNA and protein levels of CaM, CaMKⅡ, respectively, in postnatal day 1, day 7, day 14, day 21, day 30 and day 50. Results The expression of CaM and CaMKⅡwere weaker in the first day after birth, but increased rapidly at postnatal days 14-30 (P＜0.05 or P＜0.01). The expressions continued to a higher level at postnatal day 50 (the relative values of CaMmRNA and CaMKⅡmRNA were 2.242±0.154 and 2.418±0.329, respectively; the relative values of CaM protein and CaMKⅡprotein were 0.782±0.061 and 2.194±0.256, respectively)(P＜0.05 or P＜0.01), but had low expression trend compared with postnatal day 21. Conclusions There is certain time sequence in the expressions of CaM/CaMKⅡmRNA and protein in the hippocampus of rat during postnatal development. This pattern may be closely related to the change of synaptic development and brain maturity. It is also closely related to the formation of learning and memory.
关键词
钙调蛋白 /
钙/钙调蛋白依赖性激酶Ⅱ /
海马 /
认知
Key words
calmodulin /
Ca2+/calmodulin kinaseⅡ /
hippocampus /
cognition
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基金
国家自然科学基金(81271224); 武警后勤学院创新团队基金 (WHTD 201308)
