目的 探讨不同Banff分级慢性肾脏病患者肾脏中Ets-1(V-ets erythroblastosis virus E26 oncogene homolog 1)表达的差异。方法 选取在本院进行肾穿刺活检的患者60例,根据患者肾脏纤维化程度,分为Banff分级正常、轻度、中度及重度4组,每组各15例。对4组患者的性别、年龄、生化指标及病理特征进行分析,并通过免疫组化方法检测肾组织中Ets-1的表达。结果 Banff分级轻度组的BUN、UA、iPTH、尿β2-MG较正常组增高;Banff分级中度组的BUN、SCr、UA、iPTH及尿β2-MG较正常组增高;Banff重度组的MABP、BUN、SCr、UA、血磷、iPTH、尿β2-MG均较正常组明显升高,而Hb降低。Banff轻度、中度及重度组患者球性硬化、小管萎缩、间质炎性细胞浸润及间质纤维化比例均显著高于正常组。免疫组化显示Banff分级正常组、轻度组、中度组及重度组Ets-1的 A值分别为0.10±0.01、0.15±0.08、0.32±0.07、0.54±0.12,中度组、重度组较正常组升高。结论 肾间质纤维化患者Ets-1表达升高且随Banff分级程度加重而增高。
Abstract
Objective To investigate the expression of transcription factor Ets-1 in chronic renal fibrosis.Methods A total of 60 patients underwent renal biopsy from 1 May 2013 to 31 May 2015 at the Department of Nephrology in The Third Affiliated Hospital of Suzhow University were enrolled in the study and divided into four groups according to Banff scheme. Clinical and pathological characteristics of patients were investigated.The expression of transcription factor Ets-1 was assessed by immunohistochemical stainining.Results Compared with normal group,serum BUN, UA, iPTH and urineβ2-MG were significantly elevated in Banff Ⅰgroup. Serum BUN、SCr、UA、iPTH and urineβ2-MG were significantly elevated in Banff Ⅱgroup. MABP、BUN、SCr、UA、P、iPTH and urineβ2-MG were significantly elevated in Banff Ⅲgroup while Hb decreased. Percentages of global glomerulosclerosis,tubular atrophy, inflammatory cell infiltration and interstitial fibrosis significantly increased in Banff Ⅰ,Ⅱ,Ⅲgroups than in normal group. Immunohistochemical analysis showed that absorbance values of A were 0.05±0.01, 0.15±0.08, 0.32±0.07, and 0.54±0.12, respectively. Ets-1 were up-regulated in Banff Ⅱand Ⅲ groups.Conclusion Ets-1 is up-regulated in renal fibrosis and increases with Banff schems.
关键词
转录因子 /
肾间质纤维化
Key words
transcription factor /
renal fibrosis
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参考文献
[1] Oettgen P.Regulation of Vascular Inflammation and Remodeling by ETS Factors[J] .Circ Res, 2006,99(11):1159-1166.
[2] Boor P, Ostendorf T, Floege J. Renal fibrosis: Novel insights into mechanisms and therapeutic targets[J]. Nat Rev Nephrol, 2010,6(11):643-656.
[3] Hahne J C,Okudueu A F,Sahin A,et al.The transcription factor ETS-1:its role in tumour development and strategies for its inhibition[J].Mini Rev Med Chem, 2008,8(11):1095-1105.
[4] Naito T, Razzaque M S, Nazneen A, et al.Renal expression of the ets-1 proto-oncogene during progression of rat crescentic glomerulonephritis[J]. J Am Soc Nephrol, 2000,11(12):2243-2255.
[5] Kanazawa A, Tsukada S, Sekine A, et al.Association of the gene encoding wingless-type mammary tumor virus integration-site family member 5b (wnt5b) with type 2 diabetes[J]. Am J Hum Genet, 2004,75(5):832-843.
[6] Puri S, Rodova M, Islam M R, et al.Ets factors regulate the polycystic kidney disease-1 promoter[J]. Biochem Biophys Res Commun, 2006,342(4):1005-1013.
[7] Liu D X, Liu X M, Su Y, et al.Renal expression of proto-oncogene ets-1 on matrix remodeling in experimental diabetic nephropathy[J].Acta Histochem, 2011,113(5):527-533.
[8] Mizui M, Isaka Y, Takabatake Y,et al.Transcription factor Ets-1 is essential formesangial matrix remodelin[J].Kidney Int, 2006,70(2):298-305.
[9] Naito T, Razzaque M S, Nazneen A,et al.Renal Expression of the Ets-1 Proto-oncogene during Progression of Rat Crescentic Glomerulonephritis[J].J Am Soc Nephrol, 2000,11(12):2243-2255.
[10] Raffetseder U, Wernert N, Ostendorf T,et al.Mesangial cell expression of proto-oncogene Ets-1 during progression of mesangioproliferative glomerulonephritis[J]. Kidney Int, 2004,66(2):622-632.
[11] Tanaka H, Terada Y, Kobayashi T,et al.Expression and function of Ets-1 during experimental acute renal failure in rats[J].J Am Soc Nephrol, 2004,15(12):3083-3092.
[12] Naito T, Razzaque M S, Nazneen A,et al.Renal expression of the Ets-1 proto-oncogene during progression of rat crescentic glomerulonephritis[J].J Am Soc Nephrol, 2000,11(12):2243-2255.
[13] Liu D X, Liu X M, Su Y,et al.Renal expression of proto-oncogene ets-1 on matrix remodeling in experimental diabetic nephropathy[J].Acta Histochem, 2011,113(5):527-533.
[14] 袁伟杰,王 轩.Ets-1在肾脏纤维化中的双向作用及其机制[J].中国中西医结合肾病杂志, 2011,12(12):1035-1037.
[15] Di J,Jiang L,Zhou Y,et al.Ets-1 targeted by microrna-221 regulates angiotensin II-induced renal fibroblast activation and fibrosis[J].Cell Physiol Biochem,2014,34(4):1063-1074.
[16] 陈亚坤,傅淑霞,蔡澎涛.E26转录因子1与肾间质纤维化[J].临床荟萃, 2009,24 (16 ):1468-1470.
基金
常州市卫生局指导性科技项目,WZ201009
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