目的 探讨茵陈总黄酮对四氯化碳(CCl₄)致肝损伤模型大鼠的肝脏保护作用。方法 选择雄性清洁级SD大鼠112只, 实验分为6组, 即正常对照组、模型对照组、茵陈总黄酮低、中、高剂量组、阳性对照组(茵栀黄口服液组)。正常对照组12只, 其余每组20只。测定大鼠的体重和存活率, 测定大鼠血清的丙氨酸氨基转移酶(alanine aminotransferase, ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase, AST)活性、蛋白浓度及肝组织羟脯氨酸的含量, 并取肝脏组织作病理检查。结果 茵陈总黄酮高剂量组大鼠血清ALT为(2659.8±923.8) nmol/(s·L), 与模型对照组(6274.3±2018.3)nmol/(s·L)相比显著性降低, 且茵陈总黄酮各组大鼠血清ALT活性随剂量增加而降低。大鼠血清AST变化趋势与ALT相同。与模型对照组相比, 茵陈总黄酮组的总蛋白和白蛋白浓度有所增加, 而茵陈总黄酮各组肝组织病理学评分均有不同程度的降低(P<0.01), 其中高剂量组病理组织学评分为(1.92±0.79), 与模型对照组(3.86±0.43)相比降低得最明显, 此外茵陈总黄酮还能降低肝组织羟脯氨酸含量及抑制肝纤维化作用。结论 茵陈总黄酮对CCl₄所致大鼠慢性肝损伤具有保护作用。

Objective To investigate the protective effect of total flavones of Artemisia capillaris Thumb on chronic liver injury induced by subcutaneous injection of CCl₄ continually in rats. Methods The rats in experiment were divided into 6 groups as follows: normal control group (0.9% sodium chloride), model group, positive group (Yinzhihuang oral solution group), low, middle, and high dosage groups of total flavones of Artemisia capillaris Thunb. Body weight and survival rate of rats were determined. The serum sample and the liver tissue samples from all groups were collected for the biochemical and pathological examination, respectively. Results Animal serum ALT in high dose group of Artemisia capillaris Thumb flavones extract was (2659.8±923.8) nmol/(s·L). It was significantly lower when compared with the model group (6274.3±2018.3) nmol/(s·L). Serum ALT activity decreased with increasing doses of Artemisia capillaris Thumb flavones extract. Serum AST had same tendency as serum ALT. Compared with model group, concentration of total protein and albumin increased in the group of Artemisia capillaris Thumb flavones extract. Liver tissue pathology score reduced to varying degrees (P<0.01). The score in high dose group was 1.92±0.79 and it was much lower than that in model group (3.86±0.43). In addition, Artemisia capillaris Thumb flavones extract can also reduce the liver hydroxyproline content and inhibit liver fibrosis. Conclusions The total flavones of Artemisia capillaris Thumb are effective in protecting the chronic liver injury induced by CCl₄ in rats.