目的 探讨亚硒酸钠对海洛因吸食者T淋巴细胞的保护作用及其与线粒体解偶联相关指标的关系。方法 使用不同海洛因吸食年限人员外周血, 提取T淋巴细胞;四甲基偶氮唑蓝(methyl thiazolyl tetrazolium, MTT)法检测细胞生长情况, 荧光分光光度法检测细胞内活性氧(reactive oxygen species, ROS)、细胞线粒体膜电位(mitochondrial membrane potential, △Ψm), 用紫外分光光度计测线粒体通透性转运孔(mitochondrial permeability transition pore, MPTP)。结果 随着吸食海洛因的时间增加, T淋巴细胞的增殖活性下降, 差异有统计学意义(P<0.05);随着海洛因吸食年限的增加, T淋巴细胞线粒体MPTP增加、△Ψm下降、ROS升高(P<0.05);亚硒酸钠可逆转上述改变, 且存在量效关系(P<0.05)。结论 长时间吸食海洛因所造成的氧化应激状态, 通过线粒体途径对T淋巴细胞的功能和增殖情况有不利影响;亚硒酸钠可以通过其抗氧化作用, 降低ROS水平来逆转上述过程, 对T淋巴细胞具有保护作用。
Abstract
Objective To study whether or not there is protective effect of sodium selenite on T lymphocytes of heroin addicts, and if it correlates with mitochondrial uncoupling related indicators. Methods T lymphocytes extracted from peripheral blood of people who abuse heroin in different periods. Cell proliferation was detected by MTT, the levels of ROS, △Ψm were detected by fluorescence spectrophotometer, MPTP was measured by ultraviolet spectrophotometer. Results With the increased duration of heroin-abuse, T lymphocyte proliferation activity decreased significantly(P<0.05); T lymphocyte mitochondrial MPTP increased, △Ψm decreased, ROS increased(P<0.05). Sodium selenite can reverse these changes, and there is a dose-effect relationship(P<0.05). Conclusions Oxidative stress caused by prolonged heroin abuse has an adverse effect on the function and proliferation of T lymphocytes via the mitochondrial pathway. Sodium selenite has a protective effect on T lymphocytes by its antioxidant effect, which reduces the level of ROS to reverse the above process.
关键词
亚硒酸钠 /
T淋巴细胞 /
线粒体 /
线粒体通透性转运孔 /
活性氧 /
细胞线粒体膜电位
Key words
sodium selenite /
T lymphocytes /
mitochondria /
mitochondrial permeability transition pore /
reactive oxygen species /
mitochondrial membrane potential
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基金
贵州省科技术基金项目(黔科合J字[2012]2322号)
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