目的 探讨绝经前后子宫内膜息肉芳香化酶表达的异同。方法 选择临床诊断子宫内膜息肉且行宫腔镜手术的患者,按照是否绝经分为绝经前组及绝经后组,每组20例。绝经后组取其息肉组织为实验1组,取远离息肉的子宫内膜组织为对照1组;绝经前组取其息肉组织为实验2组,取远离息肉组织的子宫内膜组织为对照2组。采用免疫组化二步法和图像分析软件检测绝经前后子宫内膜息肉及远离息肉的内膜组织中芳香化酶的表达情况。结果 实验1组和2组芳香化酶的表达分别为0.1001±0.0140和0.0651±0.0098,前者大于后者,差异有统计学意义(t=9.16,P＜0.01);对照1组和2组分别为0.0269±0.0066和0.0342±0.0083,两组比较差异无统计学意义;实验1组和2组均远远高于相对应的对照1组和2组,差异有统计学意义(t=21.16,t=8.49,P均＜0.01)。结论 无论绝经前后,子宫内膜息肉中的芳香化酶的表达均高于相应生理阶段的子宫内膜中该酶的表达;绝经前后子宫内膜息肉中芳香化酶的表达不同,绝经前后子宫内膜息肉的发生可能存在不同的机制。
Abstract
Objective To investigate the differences of aromatase expression in endometrial polyps between premenopausal and postmenopausal periods. Methods According to menstrual status, the patients were divided into different groups: premenopausal group and postmenopausal group. According to the strict exclusion criteria, 20 patients were selected into each group. The postmenopausal endometrial polyps were sampled as the experiment group 1, while the postmenopausal remoteendometrial tissues not belonging to the polyp were sampled as the control group 1. The premenopausal endometrial polyps were sampled as the experiment group 2, while the premenopausal remote endometrial tissues not belonging to the polyp were sampled as the control group 2. All patients underwent hysteroscopic resection of endometrial polyps. Immunohistochemistry was used to detect the expression of aromatase in endometrial polyps and the remote endometrial tissues. Images were analyzed by using Image-Pro Plus 6.0 analysis software. The mean optical density values of aromatase expression were taken as comparison index. Results The expressions of aromatase in experiment group 1 and group 2 were 0.1001±0.0140 and 0.0651±0.0098, the differences was statistically significant (t=9.16, P＜0.01). The expressions of aromatase in control group 1 and group 2 were 0.0269±0.0066 and 0.0342±0.0083, the differences between two groups was no statistically significant. The expressions of aromatase in two experiment groups were higher than in two corresponding control groups, the differences were statistically significant (t=21.16, P＜0.01; t=8.49, P＜0.01). Conclusions The presence of aromatase expression is significantly higher in endometrial polyps than in endometriam, both in premenopausal and in postmenopausal period. The expression of aromatase in postmenopausal endometrial polyps is higher than in premenopausal endometrial polyps, so the pathogenesis of endometrial polyps may be different in the two different physiological stages.
关键词
子宫内膜息肉 /
子宫内膜 /
芳香化酶 /
绝经前 /
绝经后 /
发病机制
Key words
endometrial polyp /
endometriam /
aromatase /
premenopausual /
postmenopausal /
pathogenesis
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