目的 研究细菌鞭毛蛋白衍生物CZLC331对溃疡性结肠炎(ulcerative colitis, UC)小鼠抑郁行为的影响。方法 将18只小鼠随机分为模型组、治疗组和对照组,每组6只。模型组与治疗组首先采用2,4,6-三硝基苯磺酸(2,4,6-Trinitrobenzenesulfonic acid,TNBS)溶液灌肠的方法建立小鼠UC模型,模型组正常喂养2 d,而治疗组在造模后4 h腹腔注射3.2 mg/kg CZLC331蛋白;对照组采用380 ml/L乙醇溶液灌肠1次,正常喂养2 d。比较三组小鼠强迫游泳实验时间、小鼠悬尾实验时间、小鼠糖水偏爱百分比。结果 在强迫游泳实验和悬尾实验中,治疗组小鼠不动时间[(64.7±10.4)s,(62.5±13.9)s]均低于模型组[(86.7±16.0)s,(111.3±41.1)s],并且高于对照组[(21.0±5.3)s,(16.0±8.3)s],差异均有统计学意义(P＜0.01)。在糖水偏爱实验中,治疗组小鼠糖水偏爱(66.5±7.3)%高于模型组(34.5±4.6)%,低于对照组(77.2±7.7)%,差异均有统计学意义(P＜0.01)。结论 细菌鞭毛蛋白衍生物CZLC331可以有效改善UC小鼠的抑郁程度。
Abstract
Objective To study the effect of flagellum protein derivative CZLC331 on the depressive behavior of mice with ulcerative colitis. Methods UC mice model was established by TNBS enema. Eighteen SPF male Balb/c mice were randomly and equally divided into the control group, UC model group, and CZLC331 treatment group, six mice in each group. Mice depression index was observed by forced swimming test, tail suspension experiment and sugar water consumption experiment. Results For the forced swimming test and tail suspension test, the immobility time in treated mice [(64.7±10.4)s,(62.5±13.9)s]was significantly lower than in the model group[(86.7±16.0)s,(111.3±41.1)s], and significantly higher than in the control group[(21.0±5.3)s,(16.0±8.3)s]. In sugar water consumption experiment, the percentage of sucrose preference in treated mice (66.5±7.3)% was significantly higher than in model group(34.5±4.6)%, and significantly lower than in the control group(77.2±7.7)%. Conclusions CZLC331 can effectively improve the level of depression in mice with ulcerative colitis.
关键词
溃疡性结肠炎 /
CZLC331 /
小鼠 /
抑郁
Key words
ulcerative colitis /
cZLC331 /
mouse /
depression
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