目的 研究CD34在萎缩性胃炎、肠上皮化生、上皮内瘤变和胃癌组织中的表达水平及其临床意义。方法 采用免疫组化方法检测胃癌前病变及胃癌组织中CD34 的表达,对CD34染色阳性的微血管进行计数,研究胃癌发生发展过程中CD34表达情况,以及与胃癌临床病理学特征的关系。结果 CD34的阳性反应物质呈棕黄色细颗粒状,表现为圆形、椭圆形、条索状、分枝状及不规则形状。正常胃黏膜、萎缩性胃炎、肠上皮化生、上皮内瘤变、胃癌的微血管密度值分别为11.15±2.36、14.68±3.78、22.18±4.12、39.38±10.43、57.54±12.57。上皮内瘤变与胃癌组织微血管密度值明显高于萎缩性胃炎与肠上皮化生(P<0.05)。CD34在胃癌前病变及胃癌组织中的表达与胃癌的分化类型及淋巴结转移有关(P<0.05)。结论 CD34在胃癌组织中的表达明显高于胃癌前病变,CD34在萎缩性胃炎、肠上皮化生、上皮内瘤变及胃癌中的表达呈逐渐增多的趋势,提示CD34的异常表达与胃癌的发生发展关系密切,有望成为胃癌发生发展的预测指标。
Abstract
Objective To study the clinical significance of CD34 expression levels and in atrophic gastritis, intestinal metaplasia, intraepithelial neoplasia and gastric carcinoma. Methods Immunohistochemistry was used to detect expression of CD34 in precancerous lesions and gastric carcinoma, CD34 staining positive microvessels were counted, the occurrence and development of gastric cancer in CD34 expression and its relationship with clinicopathological features of gastric cancer were studied. Results CD34 positive reaction showed brown granular substance, expressed as round, oval, cord-like, branched and irregular shapes. The densities of microvessels in normal gastric mucosa, atrophic gastritis, intestinal metaplasia, intraepithelial neoplasia, and gastric carcinoma, were 11.15±2.36,14.68±3.78,22.18±4.12,39.38±10.43, and 57.54±12.57, respectruely. The microressel densities in intraepithelial neoplasia and gastric careinoma were significantly higher than those in atrophic gastritis and intestinal metaplasia (P<0.05). Expression of CD34 in gastric precancerous lesions and gastric carcinoma correlated with differential type of gastric carcinoma is lymph node metastasis (P<0.05). Conclusions The expression of CD34 in gastric carcinoma is significantly higher than that in precancerous lesions; Expresion of CD34 in atrophic gastritis, intestinal metaplasia, intraepithelial neoplasia and gastric cancer shows a gradually increasing trend, suggesting that abnormal expression of CD34 is closely related to the development of gastric cancer, and is expected to be a predictor of the development of gastric cancer.
关键词
胃癌 /
癌前病变 /
CD34 /
微血管密度
Key words
gastric cancer /
precancerous lesions /
CD34 /
microvessel density
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