目的 为探索胃癌分子成像技术的新方法,构建一套新型灵活的宽视场荧光内镜系统,并用于裸鼠胃癌移植瘤模型荧光成像研究。方法 通过合理的光路设计及光学组件机加工,装配宽视场荧光内镜系统,通过对不同浓度的2-DG-750荧光探针进行成像,分析系统性能;对尾静脉注射100 μl 2-DG-750荧光探针的裸鼠胃癌移植瘤模型进行在体荧光成像,对ROI的像素值和成像图进行分析。结果 体外成像ROI的像素值与荧光探针浓度存在较好的线性回归关系,在2-DG-750剂量为31.3 pmol时,该系统即可检测到荧光信号,提示该系统灵敏性好;在体成像显示用该系统联合2-DG-750荧光探针观察到了肿瘤组织的较高荧光信号强度。结论 宽视场荧光内镜系统具有很好的灵敏性,可以同时实现白光及荧光的双模式成像,具有实现光学分子成像诊断胃癌的潜在应用价值。
Abstract
Objective To setup a new flexible wide-field epi-fluorescence endoscope system, then make a fluorescence imaging for gastric cancer xenograft model in nude mice by this system, so as to setup a new device and method for the diagnosis of gastric cancer molecular imaging techniques. Methods The widefield fluorescence endoscope system was assembledusing the rational optical path and optical components machining. Different concentrations of 2-DG-750 fluorescent probe was used to analyze the performance of the imaging system.By intravenous injection of 100 μl2-DG-750 fluorescent probe into the xenografted tumor model,in vivo fluorescence imaging wasconducted, and ROI pixel values and imaging map were analyzed. Results The ROI pixel values demonstrated that a sensitivity of 31.3 pmoles 2-DG-750 was achieved as well as a good linear response from 1nmole to zero fluorescent probe. Fluorescence imaging indicated that gastrointestinal tumor tissues have an eminent 2-DG-750 uptake yielding a relatively strong fluorescence signal. Conclusions The new flexible wide-field epi-fluorescence endoscope system not only has a good sensitivity, but also can do a dual white and fluorescence imaging, which makes a promise future that optical technique will be applied to the diagnosis of gastric cancer.
关键词
荧光内镜 /
宽视场 /
近红外荧光探针 /
肿瘤检测
Key words
fluorescence endoscopy /
wide-field /
near-infrared fluorescent probe /
tumor detection
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