目的 观察模拟失重对雌性大鼠背根神经节(dorsal rootganglion, DRG)的影响。方法 选取健康雌性大鼠80只,随机分为尾部悬吊实验组(n=40)和正常对照组(n=40)。实验组按照Morey-Holton教授提出的方法保持-30°头低位及后肢自由悬垂不负重的状态,各组大鼠按标准条件进行饲养。4周后取右侧L5 DRG,HE染色观测DRG细胞形态,免疫组化观测髓鞘情况,电镜观测DRG髓鞘及线粒体超微结构。结果 HE染色结果显示,与对照组相比,实验组DRG细胞间隙轻度增宽,部分节细胞与卫星细胞分离。免疫组化结果显示,对照组免疫组化染色及IOD值(0.573±0.007)较实验组IOD值(0.802±0.009)降低(P<0.05),电镜下可见实验组髓鞘结构紊乱、松散,线粒体肿大、变形。结论 模拟失重可引起雌性大鼠DRG髓鞘及线粒体发生损伤性变化,推测模拟失重下可导致雌性大鼠DRG损伤。
Abstract
Objective To observe the effect of simulated weightlessness on DRG in female rat model. Method 80 female Sprague-Dawley rats were randomly designated as HU groups (n=40) and normal control(NC) groups (n=40). Four weeks later, the L5 DRG was excised, lumbar 5 DRG were stained with HE staining, the myelin of DRG was observed by using immunohistochemistry. TEM method aimed to observe the DRG myelin and mitochondria. Results HE staining showed that, in HU groups, the arrangement between cells in DRG were slightly loose, some ganglion cells and satellite cells were separated. IOD in NC groups was 0.573±0.007, which was lower than in HU groups (0.802±0.009)(P<0.05). In HU group, the structure of myelin was disorderly and twisted. Mitochondria in HU group were swollen and deformed obviously. Conclusions Stimulated weightlessness can cause the damage changes in the DRG myelin and mitochondria. These suggests that simulated weightlessness can cause damage in female rat DRG.
关键词
模拟失重 /
背根神经节 /
动物模型
Key words
simulated weightlessness /
dorsal root ganglion /
animal model
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基金
航天医学基础与应用国家重点实验室开放基金(SMFA15K03);武器装备预研基金(9140A17040109JB1003)
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