目的 通过对视网膜母细胞瘤(retinoblastoma, RB)移植瘤小鼠进行青蒿琥酯(artesunate, ART)联合卡铂治疗,并与单独采用卡铂、ART治疗组分别进行比较,以观察ART联合卡铂在RB治疗中的协同作用。方法 (1)将浓度为3.5×107/ml RBY-79肿瘤细胞注入新生小鼠右眼玻璃体内,建立RB移植瘤小鼠模型。(2)将建模成功的RB移植瘤小鼠随机分为4组,实验组为ART联合卡铂组;对照组分3组,分别为卡铂组、ART组、空白对照组,对各组移植瘤小鼠分别给药,观察肿瘤变化及移植瘤小鼠生存时间,进行组间比较,并观察ART联合卡铂的协同作用。结果 (1)成功建立RB移植瘤小鼠模型,移植瘤小鼠右眼重量明显大于正常左眼(P<0.0001)。(2)与空白对照组相比,联合用药组、卡铂组、ART组移植瘤小鼠的肿瘤均明显缩小,P值分别为<0.0001,<0.0001,0.0015;联合用药组、卡铂组移植瘤小鼠生存时间明显延长,P值分别为0.0253,0.0253;ART组移植瘤小鼠生存时间较空白对照组无明显延长,P值为0.8722。(3)与ART组相比,联合用药组及卡铂组移植瘤小鼠的肿瘤均明显缩小,P值分别为0.0084及0.0015;联合用药组与卡铂组移植瘤小鼠生存时间明显延长,P值分别为0.0240,0.0239。(4)联合用药组与卡铂组相比,移植瘤小鼠的肿瘤无明显缩小(P=0.7980);移植瘤小鼠生存时间无明显延长(P=1.0000)。结论 ART、卡铂及两药联合对RB均有明显抑制作用,但ART联合卡铂对RB无协同治疗作用。
Abstract
Objective This study was designed to determine the combined therapeutic effects of artesunate and carboplatin in treating retinoblastoma, compared with carboplatin alone, artesunate alone and control groups after having established newborn mice retinoblastoma model successfully. Methods (1)The cell line RBY-79 in a single-cell suspension was implanted into the right vitreous body of newborn littermates to set up a retinoblastoma xenograft model.(2)Mice bearing palpable tumors were randomly divided into four independent groups after all retinoblastoma xenograft models had been established, respectively, combined group, carboplatin group, ART group and control group. Our aims were to observe and compare the tumor size and survival time of retinoblastoma newborn mice in different groups. Results (1)Retinoblastoma xenograft models of newborn mice were successfully established and a consistent and statistically significant difference was found in the tumor weight between the right and left eyes (P<0.0001).(2)①The tumor weights in combined group, carboplatin group and ART group were significantly less than that in control (P<0.0001,P<0.0001,P=0.0015), at the same time, the survival time in these the former two groups were longer (P=0.0253,P=0.0253), that in ART group was not longer(P=0.8722). ②The tumor weights in combined group, carboplatin group were significantly less than that in ART group (P=0.0084, P=0.0015), and the survival time in the two groups were longer (P=0.0240, P=0.0239). ③The tumor weight in combined group was significantly no less than that in carboplatin group and the survival time was not longer (P=0.7980, P=1.0000). Conclusions In our study, we can find that combined treatment, carboplatin and ART can significantly inhibit the growth of retinoblastoma in vivo. However, ART does not significantly enhance the antitumor effects of carboplatin during the treatment.
关键词
视网膜母细胞瘤 /
青蒿琥酯 /
卡铂 /
联合用药
Key words
retinoblastoma /
artesunate /
carboplatin /
combined treatment
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参考文献
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基金
首都临床特色应用研究专项(Z121107001012055)
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