目的 探讨PAA-CS超吸水海绵应用于口腔拔牙创的优势。方法 建立对照组(A组)SD大鼠拔牙创模型和华法林组(B组)SD大鼠凝血功能异常拔牙创模型,以纱布棉球做阴性对照对拔牙创进行止血,比较PAA-CS超吸水海绵与纱布棉球的止血效果。结果 B组凝血酶原时间(s)较A组显著延长[(19.73±2.74)vs(10.04±0.95),P<0.05],INR值增大(3.49 vs 1)。A、B两组中,PAA-CS超吸水海绵止血时间均少于纱布棉球,差异有统计学意义(P<0.05);A组止血后无继发出血;B组应用纱布棉球止血后有4只继发出血。结论 成功建立了因喂服华法林而导致凝血功能异常的大鼠拔牙创模型,PAA-CS超吸水海绵的止血效果明显优于纱布棉球。
Abstract
Objective To observe hemostatic effects of PAA-CS(poly(acrylic acid)-chitosan) superabsorbent sponge on SD rats fed on warfarin after their tooth extraction, discussing the advantages of PAA-CS superabsorbent sponge applied for tooth extraction wounds.Methods Group A,a model of tooth extraction wounds of SD rats, and Group B,a model of tooth extraction wounds of SD rats with coagulation function abnormality, were established to carry out hemostasis on tooth extraction wounds with a negative control group using gauze tampons. Hemostatic effects of PAA-CS superabsorbent sponge and gauze cotton balls were compared.Results By four coagulation tests, the coagulation function of rats in Group B became abnormal. During the examination of hemostasis of tooth extraction wounds, the time used for hemostasis by the PAA-CS Superabsorbent Sponge(about 60 s) was significantly less than that by gauze tampons(>90 s,P<0.01). After the PAA-CS superabsorbent sponge was used to stop bleeding of tooth extraction wounds in the bleeding model of rat tooth extraction wounds, there was no secondary hemorrhage and its hemostatic effect was reliable. Conclusions This examination established a model of tooth extraction wounds of rats fed on warfarin resulting in abnormality of rats’ coagulation function successfully. The hemostatic tests have proved that the hemostatic effect of PAA-CS superabsorbent sponge is much better than that of gauze cotton balls.
关键词
PAA-CS超吸水海绵 /
华法林 /
拔牙创止血
Key words
PAA-CS superabsorbent sponge /
warfarin /
hemostasis on tooth extraction
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参考文献
[1] Napeas J J, Oost C D, Groot A, et al. Review of postoperative bleeding risk in dental patients on antiplatelet therapy[J]. Oral surgery, oral medicine, oral pathology and oral radiology, 2013, 115(4): 491-499.
[2] Lillis T, Ziakas A, Koskinas K, et al. Safety of dental extractions during uninterrupted single or dual antiplatelet treatment[J]. Am J Cardio, 2011,108(7):964-967.
[3] Alam H B,Chen Z,Jaskille A,et al.Application of a zeolite hemostatic agent achieves 100％ survival in a lethal model of complex groin in jury in Swine[J].J Trauma, 2004, 56:974-983.
[4] 隋永芳,程 刚,邹梅娟, 等.大鼠体内格列齐特对华法林钠药效学和药动学的影响[J].中国药剂学杂志,2010,8(4):110-115.
[5] 王 秀,夏 泉,许杜娟.莪术中莪术二酮抗凝血和抗血栓作用的实验研究[J]. 中成药杂志,2012,34(6):550-553.
[6] 汪 林.Expression and the role of the IR and IGF-IR in the healing process of tooth extraction socket in GK rats[D].北京:军医进修学院解放军总医院,2010:31.
[7] Rodríguez C A, Barona D C, Leco B I, et al. Extractions without eliminating anticoagulant treatment: a literature review[J]. Med Oral Patol Oral Cir Bucal, 2011, 16:800-804.
[8] Haaparanta A M, Jrvinen E, Cengiz I F, et al. Preparation and characterization of collagen/PLA, chitosan/PLA, and collagen/chitosan/PLA hybrid scaffolds for cartilage tissue engineering[J]. J Mater Sci: Mater Med, 2014,25(4):1129-1136.
[9] Smirnou D, Krcmar M, Prochazkova E. Chitin-glucan complex production by schizophyllum commune submerged cultivation[J]. Polish J Microbio, 2011, 60(3): 223-228.
[10] Foerch C, Arai K, Vancott E M, et al. Rapid reversal of anticoagulation reduces hemorrhage volume in a mouse model of warfarin-associated intracerebral hemorrhage[J]. J Cereb Blood Flow & Metabolism, 2009, 29(5): 1015-1021.
[11] Foerch C, Arai K, Jin G, et al. Experimental model of warfarin-associated intracerebral hemorrhage[J]. Stroke, 2008, 39(12): 3397-3404.
基金
武警总医院二类课题基金项目(WZ20130209)
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