目的 评估miRNA-214介导电针预处理对大鼠心肌缺血-再灌注损伤的保护作用。方法 雄性SD大鼠24只随机分为四组(n=6):假手术组(Sham组)、Sham+电针组(sham+EA)、缺血-再灌注组(I/R组)、缺血-再灌注+电针组(I/R+EA组)。Sham+EA组、I/R+EA组在心肌缺血-再灌注损伤实验前3 d，每天给电针预处理。I/R组及 I/R+EA组行心肌缺血-再灌注造模。监测Sham组、sham+EA组、I/R组及 I/R+EA组大鼠的心功能指标，并测量心肌梗死面积。检测乳酸脱氢酶、肌酸激酶活性，以及心肌细胞中miRNA-214的表达情况。结果 与sham组比较，I/R组心功能指标均降低。I/R+EA组与I/R 组比较心功能指标均有所提高。I/R+EA组心肌梗死面积显著小于I/R组。而I/R组乳酸脱氢酶、肌酸激酶活性显著升高并高于I/R+EA组(P<0.05或P<0.01)。Sham组、sham+EA组 I/R组及 I/R+EA组大鼠的miRNA-214的表达分别为1、1.6、1.75和2.82，I/R组miRNA-214的表达高于sham组，电针预处理增加了sham+EA组和I/R+EA组的miRNA-214表达(P<0.01)。结论 miRNA-214表达上调机制参与电针预处理对大鼠心肌缺血-再灌注损伤的心脏保护作用。
Abstract
Objective To evaluate whether miRNA-214 mechanism was involved in the cardioprotection by electroacupuncture pretreatment of myocardial ischemia/ reperfusion injury in rats. Methods 24 male SD rats were divided into 4 groups randomly (n=6): control group (sham group), sham + electric acupuncture group (Sham+EA) group, ischemia/reperfusion group (I/R group), ischemia/reperfusion + electric acupuncture group (I/R + EA group). Rats in group sham+EA and I/R+EA received electroacupuncture stimulation 30 min per day for 3 days before myocardial ischemia/reperfusion injury. Rats in group I/R and I/R+EA received left anterior descending (LAD) occlusion for 40min and reperfusion for 180 min. The indices of the cardiac function, myocardial infarct size , LDH and CK activities as well as expression of miRNA-214 were measured. Results All the indices of the cardiac function were not significantly different between the sham and the sham+EA group. Compared with the sham group, all the indices of the cardiac function decreased significantly in I/R group (P<0.05). I/R+EA group showed that all the indices of the cardiac function increased, compared with I/R group. Myocardial infarct size in I/R+EA group significantly decreased, compared with I/R group. The activity of LDH and CK in of I/R group significantly increased, compared with I/R+EA group (P<0.05 or P<0.01). The expression of miRNA-214 in sham, sham+EA, I/R and I/R+EA group were 1, 1.6, 1.75 and 2.82, separately. The expression of miRNA-214 in the I/R group was higher than in sham group. Electroacupuncture pretreatment increased miR - 214 expression in sham + EA group and the I/R + EA group. Conclusions Electroacupuncture pretreatment can protect the heart against myocardial ischemia/ reperfusion injury and can promote the expression of miRNA-214 in rats.
关键词
心肌缺血-再灌注损伤 /
miRNA-214 /
电针 /
保护作用
Key words
I/R injury /
miRNA-214 /
electroacupuncture /
protective effect
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基金
海南省自然基金项目(编号:20158326)
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