目的 探讨应用高压氧对阿尔茨海默病模型小鼠认知功能的影响及相关机制,为治疗阿尔茨海默病提供参考。方法 将36只KM雄性小鼠随机分成正常对照组、阿尔茨海默病模型组和造模后高压氧处理组。用D-半乳糖(120 mg/kg)和亚硝酸钠(90 mg/kg)制作阿尔茨海默病小鼠模型,利用Morris水迷宫试验进行认知功能检测,并检测小鼠海马中的SOD、GSH-Px活性及 MDA含量。结果 与正常对照组小鼠相比较,阿尔茨海默病模型组小鼠逃避潜伏期明显延长、跨越平台次数明显减少(P< 0.01);与高压氧处理前组相比,高压氧处理后组小鼠的逃避潜伏期缩短、跨越平台次数增多,均存在着统计学差异(P<0.01)。与正常对照组小鼠相比较,阿尔茨海默病模型组小鼠海马中SOD和GSH-Px活性显著降低,MDA含量显著升高(P<0.01);与高压氧处理前相比,高压氧处理后,阿尔茨海默病模型小鼠海马SOD和GSH-Px活性显著升高,MDA含量显著降低,差异均有统计学意义(P<0.01)。结论 高压氧治疗可以改善阿尔茨海默病小鼠认知功能,其机制可能与高压氧治疗可以显著提高机体抗自由基损伤能力有关。
Abstract
Objective To investigate the effect of hyperbaric oxygen on cognitive functions of mice with Alzheimer’s and related mechanisms in order to contribute to the treatment of Alzheimer’s disease.Methods 36 KM mice were randomly divided into three groups: normal control group, dementia model group, and hyperbaric oxygen treatment group. D-galactose(120 mg/kg)and sodium nitrite (90 mg/kg)were used to establish a mouse model of Alzheimer’s. The cognitive functions of mice were determined by Morris water maze, the mice’s hippocampus SOD and GSH-Px activity. The MDA level was also determined.Results Compared with the normal control group, the escape latency of the dementia model mice were prolonged, but the times of spanning the platform were significantly reduced (P<0.01). After hyperbaric oxygen treatment, escape latency was shortened while the times of spanning the platform increased. The difference was of statistical significance (P<0.01). Compared with the control group, the hippocampus SOD and GSH-Px activity in dementia model group were significantly reduced, but the MDA level was significantly increased (P<0.01). Afterhyperbaric oxygen treatment, the hippocampus SOD and GSH-Px activity of dementia model mice were significantly increased while the MDA level was significantly reduced. There were significant differences (P<0.01).Conclusions Hyperbaric oxygen therapy can improve cognitive functions in mice with Alzheimer’s, which is possibly because hyperbaric oxygen can significantly improve the body’s ability to combat free radical damage.
关键词
高压氧 /
阿尔茨海默病 /
认知功能 /
氧化应激
Key words
hyperbaric oxygen /
Alzheimer disease /
cognitive function /
oxidative stress
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