目的 探讨肿瘤坏死因子α(tumor necrosis factor,TNF-α)基因启动子区-308G/A、-857C/T位点,白细胞介素6(interleukin-6,IL-6)基因启动子-174G/C和-572C/G多态性与重度抑郁障碍症发生的相关性。方法 采用聚合酶链反应－限制性片段长度多态性(PCR-RFLP)分析方法检测393例重度抑郁障碍症患者和393名健康者对照各个多态性位点的基因型,采用SPSS 13.0进行统计学分析。结果 TNF-α基因启动子-857C/T位点的基因型及等位基因频率分布在重度抑郁障碍症组与正常对照组间存在统计学意义(P<0.05)。其中,-857T的等位基因频率只在男性重度抑郁障碍症组与对照组间存在显著差异(OR=0.309,95% CI:0.218～0.439, P<0.01),而-308A位点的等位基因频率只在女性病例组与对照组间存在统计学意义差异(OR=0.399,95% CI:0.246～0.648, P<0.01)。结论 证实TNF-α基因启动子区多态性可能是中国北方汉族重度抑郁障碍患者的风险因素。TNF-α基因启动子区多态性可能与重度抑郁障碍的发病存在关联。
Abstract
Objective To investigate the relationship between the -308G/A and -857C/T of tumor necrosis factorαgene(TNF-α),-174G/C and-572C/G of interleukin-6 gene(IL-6)polymorphisms and major depressive disorder.Methods Genomic DNA was isolated from the venous blood leukocytes of 393 unrelated patients with major depressive disorder and 393 healthy unrelated individuals(control group).All of the polymorphisms were genotyped by PCR-restriction fragment length polymorphisms (PCR-RFLP).Genotype and allele frequencies were analyzed using SPSS 13.0 software. Results There were significant differences in both allele and genotype frequencies of -857C/T of TNF-αgene between the major depressive disorder and control groups(P<0.05).The allele T of -857 T in male major depressive disorder group was significantly higher than that in contol group(OR=0.309,95% CI:0.218-0.439, P<0.01) The allele A of -308 A in female major depressive disorder group was significantly higher than that in contol group (OR=0.399,95% CI:0.246-0.648, P<0.01). Conclusions This study confirm that TNF-α gene promoter region gene polymorphism may be risk factors for severe depressive disorder in North China Han.TNF-α gene promoter polymorphism may be associated with the onset of severe depressive disorder.
关键词
重度抑郁障碍症 /
肿瘤坏死因子α /
白细胞介素6 /
遗传多态性
Key words
major depressive disorder /
tumor necrosis factor /
interleukin-6 /
genetic polymorphism
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