目的 观察不同训练方式对武警战士血浆肌酸激酶(CK)和血浆游离DNA(cf-DNA)的影响,探寻军事训练过程中早期诊断运动性疲劳和运动性肌肉损伤的敏感标志物。方法 45名某部队武警战士随机分为3组,分别为有氧训练组(5 km跑)、向心力量训练组(俯卧撑和仰卧起坐)和离心力量训练组(原地蹲跳和蛙跳),每组各15人。于训练前、训练后即刻、训练后30 min、1 h、12 h和24 h测定血浆CK和cf-DNA。结果 有氧训练组各时间点血浆CK和cf-DNA均无显著性变化(P>0.05)。向心力量训练组血浆CK在训练后12 h显著升高[(315±95) vs (134±45) U/L,P<0.01],24 h即恢复至安静水平[(151±71) vs (134±45) U/L,P>0.05];cf-DNA在训练后即刻显著升高[(59.1±12.7) vs (43.4±8.0) pg/μl,P<0.05],30 min达峰值[(95.3±13.6) vs (43.4±8.0) pg/μl,P<0.01],12 h即恢复至安静水平[(47.7±10.9) vs (43.4±8.0) pg/μl,P>0.05]。离心力量训练组血浆CK与cf-DNA的变化与向心力量组基本一致,但各时间点升高幅度均高于向心力量组(P<0.01)。结论 cf-DNA对运动的反应具有训练方式依赖性,中等强度有氧训练对cf-DNA的影响不大,力量训练特别是离心力量训练对cf-DNA的影响最大;cf-DNA可能是军事训练中早期诊断运动性疲劳和运动性肌肉损伤的敏感标志物。
Abstract
Objective To study the effect of different training modes on plasma creatine kinase (CK) and cell-free plasma DNA (cf-DNA) in soldiers of PAP and sought sensitive marker of early diagnosis of exercise-induced fatigue and muscle injury in military training. Methods Forty-five soldiers of PAP were randomly divided into three groups: aerobic training group (5 km running), concentric strength training group (push-up and sit-ups) and eccentric strength training group (squatting-jump on-the-spot and leapfrog). Plasma creatine kinase (CK) and cf-DNA were determined pre-training, immediate post-training, 30 min, 1 h, 12 h and 24 h after training, respectively. Results Plasma CK and cf-DNA in aerobic training group were not changed significantly at all time-points (P>0.05). Plasma CK of concentric strength training group was significantly elevated 12 h post-training [(315±95) vs (134±45) IU/L, P<0.01] and recovered at 24 h [(151±71) vs (134±45) IU/L, P>0.05]; cf-DNA was significantly raised immediately after training [(59.1±12.7) vs (43.4±8.0) pg/μl, P<0.05], reached peak value 30 min post-training [(95.3±13.6) vs (43.4±8.0) pg/μl, P<0.01] and restored to base-line at 12 h [(47.7±10.9) vs (43.4±8.0) pg/μl, P>0.05]. Time course changes in plasma CK and cf-DNA of eccentric strength training group were similar to those in concentric strength training group, but elevation extent was significantly higher than that in latter (P<0.01). Conclusions The response of cf-DNA to exercise is dependent on the training modes. Aerobic training of middle intensity does not influence cf-DNA but strength training especially eccentric strength training can significantly impact cf-DNA. cf-DNA may be a sensitive marker for early diagnosis of exercise-induced fatigue and exercise induced muscle injury in military training.
关键词
军事训练 /
训练方式 /
战士 /
肌酸激酶 /
血浆游离DNA
Key words
military training /
training modes /
soldier /
creatine kinase /
cell-free plasma DNA
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