目的 探讨经骨髓间充质干细胞诱导分化的神经样细胞对大鼠脊髓损伤的修复作用。方法 提取、培养和扩增骨髓间充质干细胞后,在体外诱导分化为神经样细胞。大鼠脊髓损伤造模后,分别进行神经样细胞、骨髓间充质干细胞及无细胞悬液移植,每组18只。术后采用BBB评分及斜板实验观察3组大鼠的运动功能恢复情况。结果 体外诱导骨髓间充质干细胞后,经免疫组化染色发现具备神经细胞特征。术后6周,BBB评分方面,实验组(16.56±2.42)优于阳性对照组(13.70±2.66),后者又优于阴性对照组(8.37±2.35)。Rivlin斜板实验评分方面,实验组术后2、4、6周,均优于阳性对照组,后者又均优于阴性对照组。实验组术后可见较多的神经纤维,结构完整,优于阳性对照组及阴性对照组。结论 体外诱导骨髓间充质干细胞向神经样细胞分化后,移植治疗大鼠脊髓损伤具有良好效果。
Abstract
Objective To study transplantation of bone mesenchymal stem cells (BMSCs) derived neuron-like cells for repairing the rat spinal cord injury. Methods BMSCs were isolated from the femur of 4-week-old Sprague-Dawley rats, after culture and proliferation, then differentiated into neuron-like cells induced by retinoic acid (RA). After spinal cord injury (SCI) model preparation, forty-eight rats were randomly assigned to the following three groups (n=18): the neuron-like cells group, the BMSCs group and the control group. The rats’ motor functional recovery was evaluated by BBB score and Rivlin platform test at 2 w, 4 w, 6 w after operation. Results The neuron-like cells derived from BMSCs had some neurocyte characteristics. Transplantation of neuron-like cells(16.56±2.42) showed higher score than BMSCs(13.70±2.66). The rat functional recovery in the neuron-like cells group and the BMSCs group was better than in the control group. There was more nerve fibers with normal structure could be observed in the neuron-like cells group. Conclusions Transplantation of neuron-like cells derived from BMSCs for treating rat spinal cord injury is a promising treatment.
关键词
骨髓间充质干细胞 /
神经样细胞 /
分化 /
脊髓损伤
Key words
BMSCs /
neuron-like cells /
differentiation /
spinal cord injury
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