目的 筛选积雪草苷非离子表面活性剂囊泡的最优处方和最佳制备工艺。方法 采用薄膜蒸发法制备积雪草苷囊泡，以包封率和载药量为指标对非离子型表面活性剂的种类和用量、投药量、胆固醇用量、孵化时间、孵化温度等处方因素和工艺条件进行单因素考察，正交设计筛选处方、优化制备工艺，并对积雪草苷囊泡的最优处方和工艺进行验证。结果 因素考察试验中，对积雪草苷囊泡包封率和载药量影响的顺序依次为:投药量>孵化温度>孵化时间。优化处方及工艺为:积雪草苷36 mg，孵化温度30 ℃，孵化时间30 min，其包封率和载药量分别为89.56%和25.26%，镜下观察成典型囊形。结论 采用薄膜蒸发法所制备的积雪草苷囊泡呈圆形，分散性好，囊泡的包封率和载药量均较高。

Objective To optimize the formulation and preparation technique of asiaticoside non-ionic surfactant vesicles.Methods The thin film evaporation method was used to prepare the asiaticoside niosomes, then taking encapsulation efficiency and drug loading rate as an indicator, a single factor experiment was conducted including prescription and technique factors such as the type and amount of surfactant, dosage of the drug, drug-lipid ratio, incubation time, incubation temperature, speed of the rotary evaporator and so on. The optimized asiaticoside niosomes formulation and preparation process was acquired by orthogonal method and was further verified.Results The results of single factor study showed that the three factors which had greater impact on encapsulation efficiency and drug loading rate were surfactants and the cholesterol ratio (m/m), incubation temperature and incubation time. The optimized asiaticoside niosomes formulation is as follows: asiaticoside dosage is 36 mg with the ratio of lipids as 4∶1, incubation temperature is 30 ℃, incubation time is 30 min.The encapsulation efficiency and drug loading rate of the asiaticoside noisome which was prepared according to the optimized formulation was 89.56% and 25.26% and with a microcosmic morphology of typical smooth vesicles.Conclusions The asiaticoside niosomes prepared by thin film evaporation method shows a well-rounded appearance with good dispersity and rather high encapsulation efficiency and drug loading rate.