目的 探讨单胺氧化酶A(MAO-A)基因启动子30-bp重复序列多态性与重度子痫前期发病机制的关系。方法 采用聚合酶链式反应限制性片段长度多态性(PCR-RFLP)技术检测60例重度子痫前期患者(病例组)及70例正常孕妇(对照组)MAO-A基因启动子区的基因型，并对该多态性进行统计分析。结果 MOA-A基因启动子区H/H、H/L、L/L各基因型的分布频率和H、L等位基因的分布频率在病例组与对照组之间的分布均无统计学意义(P>0.05)。结论 MAO-A基因启动子区的基因多态性可能与重度子痫前期的发病无相关性，但从机制上推测其基因多态性导致酶活性下降、儿茶酚胺灭活减少可能是重度子痫前期发病的重要原因。
Abstract
Objective To analyze the impact of this polymorphism of monoamine oxidase A(MAO-A) variable number tandem repeat (VNTR) on the risk of severe preeclampsia.Methods Sixty pregnant women wih severe preeclampsia and seventy normal pregnant women were genotyped for MAO-A VNTR polymorphism using PCR-based restriction fragment length polymorphism method.Results Significant differences were not observed between the case group and normal controls in the frequencies of MAO-A VNTR alleles and genotypes.Conclusions There is no significant difference in the allele and genotype frequencies of MAO-A gene between case group and normal controls . Our results show that MAO-A VNTR polymorphism perhaps can no increase the risk of severe preeclampsia.
关键词
重度子痫前期 /
单胺氧化酶A /
基因多态性
Key words
severe preeclampsia /
MAO-A /
gene polymorphism
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Zhang W Y,Wang L,Liu L L.The study of levels of norepinephrine and dopamine-β-hydroxylase in patients with pregnancy-induced hypertension[J]. Chin Med l J,2001,64(6):351- 356.
[2] Kaaga R J,Leinonen A,Moore P,et al. Effect of changes in body posture on vaso-active homones in pre-eclamptic women[J]. Journal of human hypertension,2004,18(11):789-794.
[3] 王 丽,张为远.正常妊娠和妊娠高血压综合征母婴儿茶酚胺水平测定[J].中华妇产科杂志,2000,35(4):233-234.
[4] Sabol S Z, Hu S, Hamer D.A functional polymorphism in the monoamine oxides A gene promoter[J].Hum Genet, 1998,103(3):273-279.
[5] 谢 幸,苟文丽.妇产科学[M].8版.北京:人民卫生出版社,2013: 64-73.
[6] 张 芸,明庆森,朱熊兆,等.单胺氧化酶A基因多态性与儿童期虐待对青少年攻击行为的影响[J].中华精神科杂志,2014,47(2):84-89.
[7] 张丽红,郭 瑶,姚常柏,等.颈交感神经干离断对妊娠期高血压疾病大鼠儿茶酚胺水平的影响[J].中国医科大学学报,2006,35(1):5-7.
[8] Hernandez M, Hernandez I, Rodriguez F,et al.Endothelial dysfunction in gestational hypertension induced by catechol-O-methyltransfense inhibition[J].Exp Physiol,2013,98(3):856-866.
[9] Markandu N D,He F J,Papavasileiou M V,et al. Urinary catecholamines and the relationship with blood pressure and pharmacological therapy[J].Journal of hypertension,2016,34(4):704-709.
基金
河北省秦皇岛市科技支撑项目(编号201302A086)
PDF(661 KB)
