目的 探讨干预FoxM1表达对食管癌细胞增殖、细胞周期及凋亡的影响。方法 选择FoxM1高表达食管癌细胞株,构建FoxM1-shRNA干扰质粒转染食管癌细胞,观察干预FoxM1表达对食管癌细胞增殖、细胞周期及凋亡的影响。结果 成功转染后细胞中FoxM1蛋白水平表达明显降低(t=13.17, P<0.01),细胞增殖明显受抑并呈时间依赖性,72 h抑制效果最显著(68.0%±6.4%,P<0.05);干预组细胞增殖周期发生G1期阻滞(59.14%±1.69% vs 40.51%±1.45%,t=14.23,P<0.01)、细胞凋亡增加(2.48%±0.49% vs 35.37%±0.56%,t=76.56,P<0.01)。结论 干预FoxM1表达使细胞周期发生G1期阻滞并能促进细胞凋亡,从而抑制TE1细胞增殖,FoxM1可能是食管癌基因治疗潜在的有效靶点。
Abstract
Objective To investigate the effects of down-regulation of forkhead box protein M1 (FoxM1) expressions by shRNA on proliferation and apoptosis of esophageal squamous cell carcinoma (ESCC) cells.Methods Plasmid that highly expressed FoxM1-shRNA was successfully constructed and transfected with TE1 cell line. FoxM1 was analyzed by Western blot. Cell proliferation was tested by cell counting kit-8 while cell cycle and apoptosis were analyzed by flow cytometry and Annexin-V-PE/7-AAD staining kit.Results FoxM1 expressions in ESCC cells transfected with specific shRNA were significantly down-regulated at the protein level and cell proliferation was inhibited in a time-dependent manner. The inhibition rate was 68.0%±6.4% (P<0.05), with cell cycle arrested at G1 phase arrest (59.14%±1.69% vs 40.51%±1.45%,t=14.23,P<0.01)and the apoptosis rate of TE1 cells reached 35.37% (2.48%±0.49% vs 35.37%±0.56%,t=76.56,P<0.01).Conclusions Interventions with FoxM1 gene expressions can inhibit the proliferation and apoptosis of ESCC cells, suggesting that FoxM1 can be a novel therapeutic target for ESCC.
关键词
食管癌 /
叉头框转录因子M1 /
基因沉默 /
细胞增殖 /
细胞凋亡
Key words
esophageal squamous cell carcinoma /
forkhead box protein M1 /
gene silencing /
cell proliferation /
apoptosis
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参考文献
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基金
基金资助:2016年湖北省教育厅科学研究计划指导性项目(B2016120),2015年随州市科研立项(2015SMR003),2016年随州市科研立项(2016SZ32005)
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