目的 研究嗅鞘细胞(olfactory ensheathing cells, OECs )移植与α晶体蛋白联合对大鼠视神经损伤后视功能恢复的促进作用。方法 玻璃体腔α晶体蛋白注射,联合视神经损伤近端移植OECs,分为4个实验组,包括α晶体蛋白注射组、OECs移植组、α晶体蛋白注射+OECs移植组、PBS对照组。于视神经损伤并移植OECs+玻璃体腔注射药物后即刻、1周、2周、1个月、2个月、3个月,用全自动眼电生理仪进行闪烁视觉诱发电位(flash-elicited visual-evoked potential,FVEP)检测,分析比较各时间点各组P1波的振幅及潜时。结果 OECs移植组及α晶体蛋白注射+OECs移植组FVEP的P1波振幅于视神经损伤后1周【(8.49±1.19)μv, (9.33±2.54)μv】、2周【(8.85±1.92)μv,(9.43±2.41)μv】、1个月【(8.46±1.09)μv,(8.72±1.91)μv】、2个月【(8.12±1.41)μv,(8.48±1.67)μv】、3个月【(7.68±2.56)μv,(8.08±1.47)μv】,均显著高于对照组(P<0.01),二者联合组P1波振幅恢复更为明显。3个实验组FVEP的P1波潜时与对照组比较,均无统计学差异。结论 OECs移植与α晶体蛋白均明显促进视神经损伤后FVEP电生理的恢复,其中,二者联合的作用最为明显。
Abstract
Objective To investigate the synergistic effect of olfactory ensheating cells (OECs) and α-crystallin on visual function recovery of nerve injury in adult rats.Methods α-crystallin was injected into the vitreous cavity, and OECs were transplanted to the area of optic nerve injury. The function of optic nerves was assessed by flash-elicited visual-evoked potential(FVEP) at 1 and 2 weeks, 1 and 3 months, and immediately after OECs transplantation and α-crystallin injection.Results Compared to PBS, OECs, the combination of OECs and α-crystallin promoted the recovery of the amplitude of P1 wave at 1 week 【(8.49±1.19)μv, (9.33±2.54)μv】, 2 weeks 【(8.85±1.92)μv,(9.43±2.41)μv】,1 month 【(8.46±1.09)μv,(8.72±1.91)μv】, 2 months 【(8.12±1.41)μv,(8.48±1.67)μv】 and 3 months 【(7.68±2.56)μv,(8.08±1.47)μv】 after optic nerve injury (P<0.01). The recovery of the amplitude of P1 wave was much more significantin the combination of OECs and α-crystallin group than in other groups. In α-crystallin group, the amplitude of P1 wave partly recovered after 1 and 2 weeks, but decreased after 1 month, and was not significantly different from that of the PBS group after 3 months. The latency of P1 wave did not recover in OECs group, α-crystallin group, or in the combination group.Conclusions Compared to OECs and α-crystallin alone, the effect of the combination of OECs and α-crystallin on functional recovery is significant.
关键词
α晶体蛋白 /
嗅鞘细胞 /
视神经损伤修复
Key words
α-crystallin /
olfactory ensheathing cells /
visual functional recovery
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Raisman G, Li Y. Repair of neural pathways by olfactory ensheathing cells[J]. Nat Rev Neurosci, 2007, 8(4): 312-319.
[2] Li Y, Sauve Y, Li D, et al. Transplanted olfactory ensheathing cells promote regeneration of cut adult rat optic nerve axons[J]. J Neurosci, 2003,23(21):7783-7788.
[3] Ying X, Zhang J, Wang Y, et al. Alpha-crystallin protected axons from optic nerve degeneration after crushing in rats[J]. J Mol Neurosci, 2008, 35(3): 253-258.
[4] Wang Y H, Wang D W, Wu N, et al. Alpha-crystallin promotes rat axonal regeneration through regulation of RhoA/rock/cofilin/MLC signaling pathways[J]. J Mol Neurosci, 2012, 46(1): 138-144.
[5] Munemasa Y, Kwong J M, Caprioli J, et al. The role of alphaA- and alphaB-crystallins in the survival of retinal ganglion cells after optic nerve axotomy[J]. Invest Ophthalmol Vis Sci, 2009, 50(8): 3869-3875.
[6] Wang Y H, Yin Z Q, Wang Y. Synergistic effect of olfactory ensheathing cells and Alpha-crystallin on restoration of adult rat optic nerve injury[J]. Neurosci Lett, 2016, 638(12): 167-174.
[7] Cao L, Su Z, Zhou Q, et al. Glial cell line-derived neurotrophic factor promotes olfactory ensheathing cells migration[J]. Glia, 2006, 54(6): 536-544.
[8] Li Y, Field P M, Raisman G. Regeneration of adult rat corticospinal axons induced by transplanted olfactory ensheathing cells[J]. J Neurosci, 1998, 18(24): 10514-10524.
[9] 李海生,潘家普. 视觉电生理的原理与实践[M].北京: 科学出版社, 1999: 190-223.
[10] Kocsis J D, Lankford K L, Sasaki M, et al. Unique in vivo properties of olfactory ensheathing cells that may contribute to neural repair and protection following spinal cord injury[J]. Neurosci Lett, 2009, 456(3): 137-142.
[11] Su Z, He C. Olfactory ensheathing cells: biology in neural development and regeneration[J]. Prog Neurobiol, 2010, 92(4): 517-532.
[12] Keyvan-Fouladi N, Raisman G, Li Y. Functional repair of the corticospinal tract by delayed transplantation of olfactory ensheathing cells in adult rats[J]. J Neurosci, 2003, 23(28): 9428-9434.
[13] Andley U P. Crystallins in the eye: function and pathology[J]. Prog Retin Eye Res, 2007, 26(1): 78-98.
[14] Mao Y W, Liu J P, Xiang H, et al. Human alphaA- and alphaB-crystallins bind to Bax and Bcl-X(S) to sequester their translocation during staurosporine-induced apoptosis[J]. Cell Death Differ,2004,11(5):512-526.
[15] Maier J, Dagnelie G, Spekreijse H, et al. Principal components analysis for source localization of VEPs in man[J]. Vision Res, 1987, 27(2): 165-177.
[16] Wolin M J, Lavin P J. Spontaneous visual recovery from traumatic optic neuropathy after blunt head injury[J]. Am J Ophthalmol, 1990, 109(4): 430-435.
[17] Sabel B A. Restoration of vision I: neurobiological mechanisms of restoration and plasticity after brain damage - a review[J]. Restorat Neurol Neurosci, 1999,15(2-3):177-200.
[18] Chow K L. Visual discriminations after extensive ablation of optic tract and visual cortex in cats[J]. Brain Res, 1968, 9(2): 363-366.
[19] Galambos R, Norton T T, Frommer G P. Optic tract lesions sparing pattern vision in cats[J]. Exp Neurol, 1967, 18(1): 8-25.
[20] Hubel D H, Wiesel T N. The period of susceptibility to the physiological effects of unilateral eye closure in kittens[J]. J Physiol, 1970, 206(2): 419-436.
基金
国家自然基金青年基金(31100706);中国博士后科学基金资助(2012M521872)
PDF(1385 KB)
