目的 探讨柚皮素预处理对心肌缺血/再灌注损伤大鼠能量代谢的影响。方法 将大鼠随机分为5组(n=10):假手术组、模型组,柚皮素分为三个剂量组(100、50、25 mg/kg),于建立模型前7 d开始腹腔注射给药,通过结扎冠脉30 min再灌注2 h建立心肌缺血/再灌注损伤模型;再灌注结束后,采用比色法测定血清肌酸激酶(CK)和乳酸脱氢酶(LDH)活性,采用定磷法测定心肌组织Na^＋/K^＋-ATP酶和Ca²⁺/Mg²⁺-ATP酶的活力,采用染色法测定心肌梗死面积(myocardial infarction surface, MIS)。结果 柚皮素高剂量组MIS缩小至32.91%,与模型组MIS(39.78%)比较差异有统计学意义(P<0.05);柚皮素各剂量组CK活性分别降低为658.03、650.12、621.89 U/L,与模型组(809.45 U/L)比较,差异均有统计学意义(P<0.05或P<0.01);各剂量组LDH活性分别降低为1543.08、1506.27、1326.97 U/L,与模型组(2034.56 U/L)比较差异均有统计学意义(P<0.05或P< 0.01);柚皮素各剂量组可显著升高Na^＋/K^＋-ATP酶活力为6.82、6.83、6.94 mmol Pi/(g·h),与模型组[5.54 mmol Pi/(g·h)]比较差异均有统计学意义(P<0.05);高、中剂量组可显著升高,Ca²⁺/Mg²⁺-ATP酶活力为8.42、8.97 mmol Pi/(g·h),与模型组[7.06 mmol Pi/(g·h)]比较差异均有统计学意义(P<0.05)。结论 柚皮素预处理对心肌缺血/再灌注所致心肌损伤的保护作用可能与改善心肌组织的能量代谢有关。

Objective To investigate the effect of naringenin on energy metabolism in rats with myocardial ischemia/reperfusion injury.Methods SD rats were divided randomly into sham group, model group, naringenin low dose group (25 mg/kg), naringenin medium dose group (50 mg/kg) and naringenin high dose group (100 mg/kg) (n=10). Naringenin was intraperitoneally injected once a day for 7 days before the modeling operation. A myocardial ischemia/reperfusion injury model was established by coronary artery ligation (30 min) and subsequent reperfusion (2 hours) in rats. After the modeling operation, the CK and LDH activities in sera were examined by chromatometry, Na^＋/K^＋-ATPase and Ca²⁺/Mg²⁺-ATPase activities in brain tissues were detected by phosphorus method and the myocardial infarction size (MIS) was determined by staining method.Results A high dose of naringenin significantly reduced MIS to 32.91% compared to 39.78% of model group (P<0.05). The CK activities in naringenin groups were 658.03, 650.12 and 621.89 U/L, respectively, which were significantly lower than 809.45 U/L of model group (P<0.05 or P<0.01). Naringenin significantly reduced LDH activities to 1543.08, 1506.27 and 1326.97 U/L, respectively, compared to 2034.56 U/L of model group (P<0.05 or P<0.01). Naringenin evidently increased Na^＋/K^＋-ATPase activities to 6.82, 6.83 and 6.94 mmol Pi/(g·h), respectively, compared to 5.54 mmol Pi/(g·h) of model group (P<0.05). Naringenin (100 and 50 mg/kg) significantly increased Ca²⁺/Mg²⁺-ATPase activities to 8.42 and 8.97 mmol Pi/(g·h), respectively, compared to 7.06 mmol Pi/(g·h) of model group (P<0.05).Conclusions The protective effects of naringenin pretreatment in myocardial ischemia/reperfusion injury seems to be associated with the improvement of energy metabolism.