目的 通过原子力显微镜(atomic force microscopy,AFM)观察人类表皮生长因子受体2(human epidermal growth factor receptor-2,HER-2)表达的乳腺癌细胞膜的表面形态变化,为提高乳腺癌HER-2检测的敏感性和精确性提供新方法。方法 利用AFM分别观察不同组织学类型的人类表皮生长因子受体抗体阳性表达和阴性表达的乳腺癌细胞膜表面形态特征并对特征参数平均粗糙度、平均峰高度、平均凹陷深度和表面积差值进行定量测量,采用统计学方法进行差异性分析。结果 HER-2阳性表达组的乳腺癌细胞形态和阴性表达组相比具有明显的不同:HER2阴性表达的乳腺癌细胞膜隆起较“尖锐”,隆起物高且偏细,每个单倍计数面积内的隆起物数目较多;而HER2阳性表达的乳腺癌细胞膜隆起较“粗大”,隆起物低且偏粗,每个单位计数面积内的隆起物数目明显减少。两组细胞在平均粗糙度[(21.87±2.46)/(32.65±1.03),P＜0.000]、平均峰高度[(13.94±1.01)/(31.15±3.89),P＜0.001]、平均凹陷深度[(11.09±6.36)/(33.58±3.15),P＜0.001]和表面积差值[(6.27±2.03)/(19.65±1.13),P＜0.001]方面具有明显差异(P＜0.05)。不同组织学类型的乳腺癌细胞膜表面形态变化趋势与此一致。结论 细胞膜表面结构因细胞功能状态的不同而不相同。相同肿瘤细胞在不同功能状态下,其细胞膜在形态结构上存在很大的差异,这为探讨肿瘤的发生机制、发展过程和早期诊断及细胞诊断提供依据,为提高HER-2的阳性率诊断提供新方法。
Abstract
Objective To observe the morphological changes in the surface of breast cancer cell’s membrane with different expressions of human epidermal growth factor receptor-2 under the atomic force microscope(AFM)in order to provide a new method for improving the sensitivity and accuracy of HER-2 detection in breast cancer and stimulate new ideas for early diagnosis of tumor at the sub-cellular level.Methods Beast cancer cells of different histological types were divided into two groups based on their expression levels of human epidermal growth factor receptor-2: the positive group and negative group. The surface morphology of cells’ membrane was observed with atomic force microscopy respectively. Such characteristic morphological parameters as average roughness,mean peak-height,average maximum depth and surface area difference of these cells were quantitatively measured ,and the Results were statistically analyzed.Results There was significant difference between the two groups in cellular morphology. The surface of positive cell membrane of human epidermal growth factor receptor-2(HER-2) was rough and uneven, and the tuberositas was high and thin, but the tuberositas was heavy and wide in the HER-2 negative group.There were more tuberosities in the positive group than in the negative group in each count area.The difference in morphological characteristics between the two groups was obvious in the average roughness[(21.87±2.46)/(32.65±1.03),P＜0.000],mean peak-height[(13.94±1.01)/(31.15±3.89),P＜0.001], average maximum depth[(11.09±6.36)/(33.58±3.15),P＜0.001]and surface area[(6.27±2.03)/(19.65±1.13),P＜0.001].Conclusions The surface structure of cell membrane varies with different functional states, which may give us an insight into the mechanism, development and early diagnosis of cancer. It could also provide a new method for the diagnosis of the positive rate of HER-2.
关键词
原子力显微镜 /
乳腺癌 /
人类表皮生长因子受体2 /
细胞膜 /
形态特征
Key words
atomic force microscopy /
breast cancer /
HER-2 /
cell membrane /
morphological characteristics
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基金
院级临床类推广应用项目(WZ2014042)
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