目的 探究干扰钙结合蛋白A13(calcium binding protein A13, S100A13)基因表达对甲状腺癌TPC-1细胞侵袭、迁移的影响及可能作用机制。方法 采用小RNA干扰敲减甲状腺癌TPC-1细胞S100A13基因的表达,通过细胞划痕实验和Transwell迁移实验检测S100A13对TPC-1细胞迁移能力的影响,Transwell侵袭实验检测S100A13对TPC-1细胞侵袭能力的影响,免疫印迹试验(Western Blot)检测S100A13、基质金属蛋白酶2(MMP-2)、钙黏蛋白E(E-cadherin)、波形蛋白(Vimentin)蛋白的表达。结果 与对照组相比,采用小RNA干扰的人甲状腺癌TPC-1细胞划痕宽度显著升高(25.214±2.687 vs 69.223±5.291),侵袭(0.897±0.082 vs 0.521±0.053)、迁移(1.263±0.252 vs 0.759±0.062)能力显著降低(P<0.05),细胞中MMP-2(0.523±0.062 vs 0.246±0.033)、Vimentin(0.863±0.082 vs 0.458±0.053)蛋白表达显著降低(P<0.05),E-cadherin(0.445±0.053 vs 0.755±0.078)蛋白表达显著增加(P<0.05)。结论 采用小RNA干扰S100A13表达可抑制甲状腺癌TPC-1细胞的侵袭、迁移,可能与其下调上皮间质转化和MMP-2的表达有关。
Abstract
Objective To investigate the effect of interference with the expression of calcium binding protein A13 (S100A13) on the invasion and migration of thyroid carcinoma TPC-1 cells and the possible mechanism.Methods The expression of S100A13 gene in thyroid cancer TPC-1 cells was knocked down by siRNA. The effect of S100A13 on the migration of TPC-1 cells was evaluated with the wound healing scratch test and Transwell assay. The effect of S100A13 on the invasiveness of TPC-1 cells was assessed by Transwell invasion test. The expressions of S100A13, MMP-2, E-cadherin and vimentin were detected by Western Blot.Results Compared with the control group, the silencing of S100A13 by siRNA in human thyroid carcinoma TPC-1 cells significantly increased the scratch width(25.214±2.687 vs 69.223±5.291), but the ability of invasion(0.897±0.082 vs 0.521±0.053) and migration(1.263±0.252 vs 0.759±0.062)decreased significantly (P<0.05). The levels of MMP-2 (0.523±0.062 vs 0.246±0.033)and vimentin(0.863±0.082 vs 0.458±0.053) in S100A13-treated cells were significantly decreased (P<0.05), while E-cadherin protein(0.445±0.053 vs 0.755±0.078)was significantly increased (P<0.05).Conclusions Interference with S100A13 expressions by siRNA inhibits the invasion and migration of thyroid carcinoma TPC-1 cells, which may be related to the inhibition of EMT and MMP-2.
关键词
甲状腺癌 /
S100A13 /
侵袭 /
迁移
Key words
thyroid cancer /
S100A13 /
invasion /
migration
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参考文献
[1] 邹 亮,原 皓. 甲状腺癌手术全麻时困难气道发生率与性别的关系[J]. 武警医学, 2016, 27(1):23-25.
[2] Zhong J, Liu C, Chen Y, et al. The association between S100A13 and HMGA1 in the modulation of thyroid cancer proliferation and invasion[J]. J Transl Med, 2016, 14(1): 80.
[3] Omotuyi O, Ueda H. Energetics and protomer communication in the dynamical structure of S100A13 in free and protein-bound states[J]. Mol Simulat, 2016, 42(11): 874-881.
[4] Dinets A, Pernemalm M, Kjellin H, et al. Differential protein expression profiles of cyst fluid from papillary thyroid carcinoma and benign thyroid lesions[J]. PloS one, 2015, 10(5): e0126472.
[5] Venyo K G, Fatola C O, Venyo K G. Melanoma of the Uterus: A Review of the Literature[J]. Int J Womens Health Reprod Sci, 2015, 3(1):2-12.
[6] Santos L, Brcic I, Unterweger G, et al. Hamartomatous polyposis in tuberous sclerosis complex: Case report and review of the literature[J]. Pathol Res Pract, 2015, 211(12): 1025-1029.
[7] 涂华华, 陈先祥, 程彩涛, 等. S100 钙结合蛋白 A13 和人成纤维细胞生长因子-1 在甲状腺肿瘤中的表达及临床意义[J].中华实验外科杂志, 2015, 32(2):391-393.
[8] 张 齐, 胡浩霖, 石 欣, 等. S100PshRNA 慢病毒载体的构建及其对胃癌 MGC-803 细胞生物学行为的影响[J]. 东南大学学报: 医学版, 2015,56(1):65-70.
[9] Wang X, Han J, Hardie D B, et al. The use of matrix coating assisted by an electric field (MCAEF) to enhance mass spectrometric imaging of human prostate cancer biomarkers[J]. J Mass Spect, 2016, 51(1): 86-95.
[10] Pierce A, Barron N, Linehan R, et al. Identification of a novel, functional role for S100A13 in invasive lung cancer cell lines[J]. Eur J Cancer, 2008, 44(1):151-159.
[11] Zhong J, Liu C, Chen Y, et al. The association between S100A13 and HMGA1 in the modulation of thyroid cancer proliferation and invasion[J].J Transl Med, 2016, 14(1):80.
[12] Chiappetta G, Valentino T, Vitiello M, et al. PATZ1 acts as a tumor suppressor in thyroid cancer via targeting p53-dependent genes involved in EMT and cell migration[J].Oncotarget, 2015, 6(7): 5310-5323.
[13] Han B, Cui H, Kang L, et al. Metformin inhibits thyroid cancer cell growth, migration, and EMT through the mTOR pathway[J]. Tumour Biol, 2015, 36(8):6295-6304.
[14] Huang J, Guo L. Knockdown of SOX9 Inhibits the proliferation, Invasion, and EMT in thyroid cancer cells.[J]. Oncol Res, 2017, 25(2):167-176.
[15] Sponziello M, Rosignolo F, Celano M, et al. Fibronectin-1 expression is increased in aggressive thyroid cancer and favors the migration and invasion of cancer cells[J]. Mol Cell Endocrinol, 2016,431(1):123-132.
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