目的 探讨生长分化因子11(growth differentiation factor 11, GDF11)对老龄小鼠内皮依赖性血管舒张功能的影响。 方法 选择20只18个月龄C57小鼠随机分为实验组(GDF11组,n=10)和对照组(Vehi组,n=10), GDF11组按0.3 mg/(kg·d)的剂量腹腔注射重组GDF11,Vehi组注射等量生理盐水。4周后取材,检测脂代谢相关的血液学指标,分离小鼠的胸主动脉,测定血管舒张功能和主动脉Samd2/3的磷酸化水平。 结果 与Vehi组比较,GDF11干预显著增强乙酰胆碱(Ach)诱导的内皮依赖性血管舒张功能[10-6 mol/L Ach: (37.1±5.2)% vs (59.7±4.3)%;10-5 mol/L Ach: (48.2±4.0)% vs (78.4±5.6)%;10-4 mol/L Ach: (61.1±4.7)% vs (90.2±7.4)%],降低血清中三酰甘油的水平[(76.6±11.2) vs (64.5±10.4) mg/dl],升高脂联素的浓度[(11.9±1.1) vs (14.2±1.7) μg/ml],并能提高主动脉Samd2/3的磷酸化水平[Smad2: (1.00±0.05) vs (1.78±0.10)]、Smad3 [(1.00±0.09) vs (1.57±0.12)]。 结论 GDF11对血管内皮细胞有保护作用。
Abstract
Objective To investigate the effects of GDF11 on endothelium-dependent vasodilation of the aorta in old mice.Methods Twenty 18-month-old C57 mice were randomly divided into GDF11 group [0.3 mg/(kg·d)] recombinant GDF11 intraperitoneal injection, n=10) and Vehi group (equivalent physiological saline injection, n=10). After four weeks of intervention, blood biomarkers of lipid metabolism were tested, while relaxation response and Western blot were determined in the isolated aorta.Results GDF11 intervention significantly increased endothelium-dependent vasodilation in response to acetylcholine (Ach) [10-6 mol/L Ach: (37.1±5.2)% vs (59.7±4.3)%、10-5 mol/L Ach: (48.2±4.0)% vs (78.4±5.6)%、10-4 mol/L Ach: (61.1±4.7)% vs (90.2±7.4)%], decreased triglyceride levels in serum [(76.6±11.2) vs (64.5±10.4) mg/dl], elevated adiponectin protein levels [(11.9±1.1) vs (14.2±1.7) μg/ml] and enhanced protein levels of phosphorylated Samd2/3 [p-Smad2: (1.00±0.05) vs (1.78±0.10)]、p-Smad3 [(1.00±0.09) vs (1.57±0.12)].Conclusions GDF11 can attenuate impaired endothelium-dependent vasodilation in old mice.
关键词
GDF11 /
内皮依赖性血管舒张功能 /
老龄鼠
Key words
growth differentiation factor 11 /
endothelium-dependent vasodilation /
old mice
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