目的 探讨标本在不同温度及不同保存时间的条件下,对糖化血红蛋白(HbA₁c)检测结果的影响。方法 取10例患者抗凝新鲜全血标本进行预处理(溶血处理),在室温下分装成6组,每组10个标本。A组立即检测,B、C、D、E、F组分别在分装后1、2、3、4、5 h检测。取5例全血标本分别充分混匀后,分4组保存,每组5个标本。A1组在室温(18～22 ℃)、B1组冷藏(2～8 ℃)、C1组冷冻(-18～-20 ℃)、 D1组低温冷冻(-80 ℃)条件下保存不同时间后,分别用OLIMPO进行检测。以低温冷冻组测定结果为标准,每个标本的HbA₁c测定结果都在低温冷冻组结果的±3s范围内为可接受结果。结果 溶血后不同时间HbA₁c检测结果:A组(7.98±1.61)%;B组(7.97±1.61)%;C组(7.95±1.60)%;D组(7.94±1.61)%;E组(7.77±1.61)%;F组(7.46±1.57)%。与A组比较,F组差异有统计学意义(t=1.57,P<0.05)。要使OLIMPO检测HbA₁c结果保持稳定,则标本的保存时间分别为:室温2 d,冷藏15 d,冷冻3 d;低温冷冻半年以上。结论 OLIMPO检测HbA₁c时,不同处理条件下,HbA₁c结果的稳定性差异较大。样本溶血处理后4 h内完成检测,结果较准确;如果全血标本需要保存较长的时间,则在2~8 ℃冷藏和-80 ℃条件下保存效果较好。

Objective To explore the effect of sample hemolysis time and preservation conditions on the detection of glycosylated hemoglobin (HbA₁c) by OLIMPO.Methods 1.Anticoagulant samples of fresh whole blood were taken from ten patients to be preprocessed (hemolyzed). Sixty samples of 150 μl each were collected and equally divided into 6 groups at room temperature. Group A was immediately detected, while groups B, C, D, E and F were detected 1, 2, 3, 4 and 5 hours later, respectively. 2. After 5 samples of whole blood were mixed properly, they were divided into 4 groups with 5 samples in each. Group A1 was stored at room temperature (18-22 ℃), group B1 was refrigerated (2-8 ℃), group C1 cryopreserved (-18－-20 ℃) , and group D1 was cryopreserved at a low temperature (below -80 ℃).The results of D1 were taken as the standard to detect the quality of each group with OLIMPO. The HbA1c results of each specimen were regarded as acceptable if they were within the range of ±3 compared with the results of D1.Results 1.HbA₁c results at different time points after hemolysis were (7.98±1.61)% in group A, (7.97±1.61)% in group B, (7.95±1.60)% in group C, (7.94±1.61)% in group D, (7.77±1.61)% in group E and (7.46±1.57)% in group F. There was statistically significant difference between group A and group F (t=1.57,P<0.05). 2. In order to keep HbA₁c results stable during OLIMPO test, the storage time of samples was 2D at room temperature, 15 d for cold storage, 3D for cryopreservation and more than half a year for cryopreservation at a low temperature.Conclusions When HbA₁c is detected with OLIMPO, the stability varies considerably under different processing conditions. The results are relatively accurate within 4 hours after sample hemolysis. If the whole blood sample specimen needs to be stored for a longer time (for scientific research or re examination), better preservation can be achieved at 2-8 ℃ and -80 ℃.