目的 探讨维持性血液透析终末期肾病(end stage renal disease,ESRD)患者肠道菌群的特点。方法 收集维持性血液透析的稳定ESRD患者和健康对照组受试者各22例粪便样本,提取样本DNA,进行16S rRNA基因测序,对肠道菌群的分布情况进行分析。结果 (1)维持性血液透析ESRD组观察到的各样品微生物物种量、物种多样性较健康对照组低,物种多样性在18～44岁组有统计学差异;(2)两组在纲、目、科、属、种分类水平上分别发现2、4、9、29、28个相对丰度有统计学差异的物种(P<0.05); 其中Negatibicutes纲、粪球菌属-3相对丰度维持性血液透析ESRD组低于健康对照组,而丹毒丝纲、目、科、属、种水平相对丰度维持性血液透析ESRD组高于健康对照组;(3)通过KEGG代谢途径的差异分析,推测两组间有3个存在统计学差异的代谢途径(P<0.05),其中与碳水化合物代谢途径相关基因在健康对照组粪便样品中显著富集,而与细胞运动性及药物耐药途径相关基因在维持性血液透析ESRD组粪便样品中显著富集。结论 维持性血液透析ESRD组患者肠道微生物群落多样性降低,与健康对照组比较在微生物群落相对丰度、KEGG功能预测相关基因代谢途径上存在差异。
Abstract
Objective To investigate the characteristics of intestinal microbiome in maintenance hemodialysis patients with end-stage renal disease (ESRD).Methods Fecal samples from 22 maintenance hemodialysis patients with stable ESRD and 22 healthy controls were collected. DNA was extracted from the samples and the bacterial composition was analyzed based on 16S ribosomal RNA pyrosequencing.Results (1)The microbial biomass and species diversity of group A were smaller than those of group B. Only the group aged 18 to 44 had significant difference in species diversity (P<0.05). (2)There were 2, 4, 9, 29 and 28 species with significant difference in relative abundance between the two groups at the classification levels of class, order, family, genus and species (P<0.05). The relative abundance of Negatibicutes and Coprococcus-3 in group A was lower than that of group B, but the relative abundance of Erysipelotrichia was higher in group A. (3)The difference analysis of KEGG metabolic pathways suggested that three metabolic pathways of KEGG between the two groups were significantly different (P<0.05). It was speculated that the genes related to carbohydrate metabolism pathways were significantly enriched in fecal samples of group B, while the genes related to cell motility and drug resistance pathways were significantly enriched in group A.Conclusions There is difference in microbial community diversity, relative abundance and related gene metabolic pathways predicted by KEGG function in ESRD maintenance hemodialysis patients compared to the healthy control group.
关键词
维持性血液透析 /
终末期肾病 /
肠道菌群
Key words
maintenance hemodialysis /
end-stage renal disease /
intestinal microbiome
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参考文献
[1] 国家肾脏疾病临床医学研究中心. 中国慢性肾脏病矿物质和骨异常诊治指南概要[J]. 肾脏病与透析肾移植杂志,2019,28(1):52-57.
[2] 中华医学会老年医学分会肾病学组,国家老年疾病临床医学研究中心. 老年人慢性肾脏病诊治中国专家共识(2018) [J]. 中国老年医学杂志,2018,37(7):725-731.
[3] Pahl M V, Vaziri N D. The chronic kidney disease-colonic axis [J]. Semin Dial,2015,28(5):459-463.
[4] 焦书沛,姜 晨.“肠-肾轴”理论研究现状及分析[J]. 中国中西医结合肾病杂志,2017,18(7):656-658.
[5] Tremaroli V, Backhed F. Functional interactions between the gut microbiota and host metabolism[J]. Nature,2012,489(7415):242-249.
[6] Warner B B, Deych E, Zhou Y, et al. Gut bacteria dysbiosis and necrotising enterocolitis in very low birthweight infants: a prospective case-control study[J]. Lancet,2016,387(10031):1928-1936.
[7] 金晓倩. 维持性血液透析终末期肾病患者肠道优势菌群多样性变化研究[J]. 中国微生物杂志,2015,27(5):513-516.
[8] Minamoto Y, Otoni C C, Steelman S M, et al. Alteration of the fecal microbiota and serum metabolite profiles in dogs with idiopathic inflammatory bowel disease[J]. Gut Microbes,2015,6(1):33-47.
[9] Pindjakova J, Sartini C, Lo Re O,et al. Gut Dysbiosis and adaptive immune response in diet-induced obesity vs. systemic inflammation[J].Front Microbiol,2017,8(6):1157.
[10] Weber G J, Foster J, Pushpakumar S B, et al. Altered microRNA regulation of short chain fatty acid receptors in the hypertensive kidney is normalized with hydrogen sulfide supplementation[J]. Semin Dial,2018,134(8):157-165.
[11] 李蔚然,陈 珉. 人类肠道微生物研究进展[J]. 生物学教育,2018,43(3):4-6.
[12] Vaziri N D,Liu S M,Lau W L,et al.High amylose resistant starch diet ameliorates oxidative stress, inflammation and progression of chronic kidney disease[J]. PLoS One,2014,9(12):e114881.
[13] Nallu A,Sharma S,Ramezani A,et al.Gut microbime in CKD:challenges and opportunities[J].Transl Res,2017,179(1):24-37.
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