目的 探究胎母输血综合征(fetomaternal emorrhage,FMH)患者F血红蛋白酸洗脱液温度对Kleihauer-Betke(K-B)实验准确率的影响。方法 将K-B实验中洗脱环节温度分别定于37 ℃和45 ℃进行试验,对数据进行分析后确定K-B实验的最适温度。结果 洗脱温度为37 ℃时,当样本为阴性标本时(＜1%), K-B法检测结果与标准值之间的差异无统计学意义;当标本为阳性标本时(≥1%),检测结果与标准值之间的差异有统计学意义(P<0.05)。在45 ℃条件下,无论标本为阳性还是阴性,其结果与标准值差异无统计学意义。结论 K-B法在洗脱温度为45 ℃时,结果更为准确,可以更加有效地将母血中的胎儿红细胞进行区别染色,为FMH的诊治和预后判断提供了良好的实验室数据支持。
Abstract
Objective To determine the optimum acid-elution temperature of Kleihauer-Betke (K-B) experiment for hemoglobin F detection and fetomaternal hemorrhage (FMH) evaluation.Methods Both 37 ℃ and 45 ℃ were chosen as the acid-elution temperatures in the K-B experiment, followed by head-to-head staining results comparison.Results When the elution temperature was set at 37 ℃ and specimens were negative, there was no statistically significant difference between the results of K-B experiments (< 1%) and theoretical values. However, with theoretically positive specimens (≥1%), the results of experiments were statistically different from the calculated values (P<0.05). When the elution temperature was set at 45 ℃, there was no significant difference between the K-B test results and their corresponding theoretical values, whether the specimens were positive or. negative.Conclusion When the elution temperature is set at 45 ℃ in the K-B experiment, we can more effectively and accurately distinguish the fetal red blood cells from maternal blood by the staining method. Our work provides helpful laboratory data support for the diagnosis, treatment and prognosis of FMH.
关键词
F血红蛋白 /
酸洗脱试验 /
洗脱液温度 /
胎母输血综合征 /
胎儿红细胞
Key words
hemoglobin F /
K-B experiment /
elution temperature /
fetomaternal emorrhage /
fetal red blood cells
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