目的 探讨测定不同产地玫瑰香葡萄藤茎中白藜芦醇和ε-葡萄素的含量的方法。方法 采用高效液相色谱法测定不同产地葡萄藤中白藜芦醇和ε-葡萄素的含量,并做加样回收率实验。色谱柱为COSMOSIL 5C18-MS-Ⅱ(4.6ID ×250 mm);流动相为甲醇-醋酸水溶液进行梯度洗脱,流速1 ml/min,柱温30 ℃,检测波长为254 nm和310 nm。结果 白藜芦醇在1.76～17.6 μg质量范围内与峰面积呈现良好的线性关系(R2=0.9999);ε-葡萄素在2.75～27.54 μg范围内与峰面积呈现良好的线性关系(R2=0.9999)。利用该方法测定10个样品中的白藜芦醇的含量范围在1.94～5.96 μg/g,含量的变异系数为40.64%;ε-葡萄素的含量范围在8.74～15.41 μg/g,含量的变异系数为17.67%。结论 该方法简便易行,结果准确,可作为控制葡萄藤质量的方法。
Abstract
Objective To determine the contents of resveratrol and ε-viniferin in the stems of muscat grape vines grown in different areas.Methods High performance liquid chromatography was applied to determine the contents with a mobile phase that consisted of methanol-acetic acid aqueous solution by gradient elution. The wavelength of detection was 310 nm and 254 nm respectively, the flow rate was 1 mL/min, and the column temperature was 30 ℃. Results The linear relationships of resveratrol and ε-viniferin were good in the range of 1.76 to 17.6 μg and 2.75 to 27.54 μg respectively(R2=0.9999). The contents of resveratrol and ε-viniferin determined with this method ranged from 1.94 to 5.96μg/g and 8.74 to 15.41 μg/g in ten samples, while their coefficients of variation were 40.64% and 17.67%, respectively.Conclusions This method is simple, accurate and can be used to for quality control of grape vines.
关键词
葡萄藤 /
高效液相色谱 /
白藜芦醇 /
ε-葡萄素
Key words
stems of grape vines /
high performance liquid chromatography /
resveratrol /
ε-viniferin
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