目的 探究microRNA-26b-5p(miR-26b-5p)介导高迁移率族蛋白组A1(high-mobility group A1,HMGA1)基因参与卵巢癌上皮间质转化的机制。方法 对miR-26b-5p靶基因进行预测。qRT-PCR检测组织中miR-26b-5p和HMGA1的表达量。卵巢癌细胞分组转染后,qRT-PCR和Western Blot检测细胞中相关因子的mRNA和蛋白的表达量;Transwell侵袭实验检测细胞侵袭,细胞划痕实验检测迁移能力。结果 卵巢癌组织中miR-26b-5p表达降低,HMGA1的表达升高(均P＜0.05)。miR-26b-5p mimic及sh-HMGA1组HMGA1和Vimentin表达降低而E-cadherin表达上调,且细胞侵袭和迁移降低(均P＜0.05);共转染miR-26b-5p inhibitor和sh-HMGA1,sh-HMGA1处理对上皮间质转化及细胞侵袭和迁移的抑制作用被逆转(均P＜0.05)。结论 过表达miR-26b-5p可抑制卵巢癌细胞中HMGA1和Vimentin表达并促进E-cadherin表达,降低细胞侵袭和迁移能力,从而抑制卵巢癌细胞上皮间质转化。
Abstract
Objective To explore the mechanism by which microRNA-26b-5p (miR-26b-5p) mediates high-mobility group A1 (HMGA1) in epithelial-mesenchymal transformation of ovarian cancer.Methods The target gene of microRNA-26b-5p was predicted. The expressions of miR-26b-5p and HMGA1 in tissues were detected by real-time reverse transcription PCR (qRT-PCR). After grouping and transfection of ovarian cancer cells, the expressions of mRNA and protein of relevant factors were detected by qRT-PCR and Western blot in the cells. Transwell invasion test was used to detect cell invasion and scratch test to detect migration ability, while qRT-PCR and Western blot were used to detect the expressions of E-cadherin and Vimentin genes related to epithelial-mesenchymal transformation in tumors.Results The expression of miR-26b-5p in ovarian cancer tissue decreased, while the expression of HMGA1 increased (All P＜0.05). miR-26b-5p mimic and sh-HMGA1 groups showed decreased expressions of HMGA1 and Vimentin, increased expression of E-cadherin, and reduced cell invasion and migration (All P＜0.05). Co-transfection of miR-26b-5p inhibitor and sh-HMGA1 reversed the inhibitory effect of sh-HMGA1 on epithelial-mesenchymal transformation (All P＜0.05).Conclusions Over-expression of miR-26b-5p inhibits the expressions of HMGA1 and Vimentin in ovarian cancer cells, promotes E-cadherin expression, reduces cell invasion and migration, thus inhibiting epithelial-mesenchymal transformation of ovarian cancer cells.
关键词
卵巢癌细胞 /
miR-26b-5p /
HMGA1 /
Vimentin /
E-cadherin /
上皮间质转化
Key words
ovarian cancer cell /
miR-26b-5p /
HMGA1 /
Vimentin /
E-cadherin /
epithelial-mesenchymal transformation
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