目的 探讨高强度间歇训练(high-intensity interval training,HIIT)对武警战士T淋巴细胞稳态(Th1/Th2和TH17/Treg)的影响。方法 选取23名武警男性战士分别进行急性(1次)和短期(4周、3次/周)HIIT,于1次HIIT运动前、运动后即刻、运动后60 min及4周HIIT前后取静脉血利用流式细胞术测定细胞因子IFN-γ(Th1亚群)、IL-4(Th2亚群)、IL-17A(Th17亚群)、TGF-β(Treg亚群),以及Treg细胞活化标志物CD39、LAP和GARP相对表达量(用阳性细胞率表示)。结果 1次HIIT后即刻,Th1细胞IFN-γ表达上调(P<0.05),60 min后,Treg细胞GARP、LAP和CD39表达量升高(P<0.05),IFN-γ恢复(P>0.05);IL-4在各时间点均无显著性变化(P>0.05)。4周HIIT后,Treg细胞LAP表达下调(P<0.05)。未检测到IL-17A和TGF-β表达量。结论 1次急性HIIT暂时性破坏Th1/Th2稳态并使Th1/Th2平衡向Th1漂移,运动后恢复期具有免疫抑制作用的Treg细胞被动员,其作用可能参与T细胞稳态的调节与重建。短期(4周)HIIT诱导Treg细胞免疫抑制能力减弱,但T细胞稳态平衡无显著性改变。
Abstract
Objective To investigate the effects of high-intensity interval training (HIIT) on T lymphocyte homeostasis (Th1/Th2 and Th17/Treg) in PAP soldiers.Methods Twenty-three PAP male soldiers were subjected to acute (a bout) and short-term (4 weeks, 3 times/week) HIIT respectively. Venous blood was taken and relative expressions (expressed by positive cell rate) of cytokines of IFN-γ (Th1 subset), IL-4 (Th2 subset), IL-17A (Th17 subset), TGF-β (Treg subset) and Treg activation markers of CD39, LAP and GARP were determined with flow cytometry before, immediately after, 60 min after acute HIIT and before and after chronic HIIT.Results Expression levels of IFN-γ in Th1 were upregulated (P<0.05) immediately after acute HIIT, while GARP, LAP and CD39 were elevated (P<0.05) and IFN-γ recovered (P<0.05) after 60 min of acute HIIT. IL-4 did not change at any time-point (P>0.05). After 4 weeks of HIIT, LAP in Treg decreased (P<0.05). Expressions of IL-17A and TGF-β could not be detected. Conclusions Acute HIIT can transiently perturb Th1/Th2 homeostasis and shift Th1/Th2 balance to Th1, while during recovery, immunosuppressive Treg cells are mobilized to possibly regulate and reconstruct T lymphocyte homeostasis. Short-term (4 weeks) HIIT can reduce immunosuppressive ability of Treg cells without altering T lymphocyte homeostasis.
关键词
高强度间歇训练 /
T淋巴细胞 /
Th1/Th2 /
Th17/Treg /
稳态平衡
Key words
high-intensity interval training /
T lymphocyte cell /
Th1/Th2 /
Th17/Treg /
homeostasis
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基金
武警部队后勤科研项目(WHKL15-3);武警部队后勤部卫生部科研项目(WJHQWSB2-13-05);武警后勤学院博士启动金项目(WHB201709)
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