目的 探讨鞣花酸(ellagic acid,EA)对正常小鼠肌肉功能的影响。方法 ICR雄性小鼠随机分为实验组和对照组,每组各8只。实验组每天给予10 mg/100 g体重的鞣花酸,对照组给予等体积溶剂,1次/d,灌胃21 d。利用Echo MRITM小动物体成分分析仪测定小鼠体成分,抓力仪测定小鼠四肢抓力,轮转式疲劳仪测定小鼠掉落次数,HE染色计算腓肠肌横截面积,以及利用qRT-PCR分析泛素蛋白酶通路中的MuRF1和Atrogin-1基因表达。结果 鞣花酸干预后,体成分检测两组肌肉含量、脂肪含量差异无统计学意义。与对照组相比,实验组小鼠抓力和肌纤维横截面积增加,掉落次数、MuRF1 和Atrogin-1的mRNA表达水平降低,差异均具有统计学意义(P<0.05)。结论 鞣花酸可能通过抑制蛋白质降解,增强小鼠肌肉功能,提升小鼠运动耐力,有望作为提升肌力及运动功能的植物化合物。
Abstract
Objective To observe the effect of ellagic acid(EA) on muscle function in normal mice.Methods ICR male mice were randomly divided into the control group and EA treatment group, with 8 mice in each. The EA treatment group was given 10 mg/100 g weight of EA per day while the control group was given an equal volume of solvent once daily for 21 days. The Echo MRITM was used to determine the body composition of the mice, and the grip tester was used to measure the grip strength of the mice. The times of drops were measured by the rotary fatigue meter,and the cross-sectional area of gastrocnemius muscle fibers was calculated by HE staining.qRT-PCR was used to analyze MuRF1 and Atrogin-1 gene expressions in the ubiquitin protease pathway.Results After EA treatment,there was no statistically significant difference in body composition between the two groups. Compared with the control group, the mice in the EA treatment group increased their grip strength and cross-sectional area of muscle fibers,but reduced the times of drops and the mRNA expression levels of MuRF1 and Atrogin-1.The difference was statistically significant (P<0.05).Conclusions Ellagic acid may inhibit the degradation of proteins, enhance the muscle function of mice, and improve exercise endurance in mice. It is expected to be a plant compound that improves muscle strength and exercise function.
关键词
鞣花酸 /
体成分 /
肌肉功能 /
MuRF1 /
Atrogin-1
Key words
ellagic acid /
body composition /
muscle function /
MuRF1 /
Atrogin-1
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基金
解放军总医院第三医学中心院内课题(WZ2016019)
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