目的 探讨特发性肾病综合征(INS)患儿血清及尿液中miRNA-150和miRNA-30a-5P作为临床诊断标志物的可能性。方法 收集2017-01至2019-12在武警特色医学中心诊断的93例未经治疗的INS患儿为患儿组,另选取同期性别、年龄相同的常规健康体检儿童89例为对照组,采用实时荧光定量PCR(qRT-PCR)检测miRNA-150及miRNA-30a-5P两组血清及尿液中的含量; ROC曲线等评估其对 INS 患儿的诊断价值。结果 与对照组相比,患儿组血清及尿液中miRNA-30a-5P及血清中miRNA-150水平明显偏高(P<0.05),尿液中miRNA-150水平,差异无统计学意义,其中46例在病情控制或缓解后血清及尿液中miRNA-30a-5P及血清中miRNA-150水平明显降低,差异具有统计学意义(P< 0.01);血清miRNA-150和miRNA-30a-5P诊断INS的ROC 曲线下面积(AUC)分别为0.852、0.869;尿液中miRNA-150和miRNA-30a-5P诊断INS的ROC曲线下面积(AUC)分别为 0.825、0.833。结论 INS患儿血清及尿液中miRNA-150及miRNA-30a-5P,可作为辅助诊断INS的分子标志物。
Abstract
Objective To analyze the difference of expressions of miRNA-150 and miRNA-30a-5P in serum and urine of children with idiopathic nephrotic syndrome (INS)and discuss their clinical value as a non-invasive auxiliary diagnostic marker of INS.Methods Urine samples were collected from 93 INS children (the patient group) who were diagnosed but waited to be treated in our hospital between January 2017 and December 2019 and from another 89 sex- and age-matched healthy children (the control group). The levels of serum and urinary miRNA-150 and miRNA-30a-5P were determined by quantitative real-time polymerase chain reaction (qRT-PCR) . The clinical value of serum and urinary miRNA-150 and miRNA-30a-5P in the diagnosis of children’s INS was assessed by the ROC curve.Results The levels of serum and urinary miRNA-30a-5p and serum miRNA-150 were slightly higher in the patient group than in healthy controls (P<0.05) , but the levels of urinary miRNA-150 were not. The levels of serum and urinary miRNA-30a-5P and serum miRNA-150 in the clinical remission period decreased significantly in forty-six of these cases(P<0.05) .The area under the ROC curve (AUCROC) of serum miRNA-150 and miRNA-30a-5P was 0.852 and 0.869 respectively for the diagnosis of INS children, compared to 0.825 and 0.833 for urinary miRNA-150 and miRNA-30a-5P.Conclusions The serum and urinary levels of miRNA-150 and miRNA-30a-5P in children with idiopathic nephrotic syndrome increase obviously, which may serve as new biopsy molecular markers for the auxiliary diagnosis of INS.
关键词
特发性肾病综合征 /
miRNA-150 /
miRNA-30a-5P /
分子标志物
Key words
idiopathic nephrotic syndrome /
miRNA-150 /
miRNA-30a-5P /
molecular marker
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