目的 建立一种可以同时测定参龙通络丸中两种成分(三七皂苷R1、人参皂苷Rf)含量的HPLC法。方法 采用高效液相色谱法(HPLC),Hypersil Hypersil BDS C18色谱柱(5 μm,4 mm×250 mm),乙腈-水为流动相梯度洗脱;流速1.0 ml/min;检测波长203 nm;柱温35 ℃,检测参龙通络丸中两种成分的含量。结果 三七皂苷R1样品量在0.198~9.535 μg范围(r=0.9993)、人参皂苷Rf的样品量在0.957~38.392 μg范围(r=0.9997)线性关系良好,两种成分的平均回收率分别为99.10%(RSD=1.57％,n=6)和98.76% (RSD=2.13％,n=6)。结论 本方法具有结果准确、可靠、重复性好的特点,可为参龙通络丸的质量标准制定提供科学依据。
Abstract
Objective To establish an HPLC method for simultaneous determination of Notoginsenoside R1 and ginsenoside Rf in Shenlong Tongluo pills.Methods High performance liquid chromatography was used.Hypersil Hypersil BDS C18(5 μm,4 mm×250 mm) column was selected with acetonitrile-water as the mobile phase for gradient elution.The flow rate was 1.0 ml/min,detection wavelength was 203 nm and the column temperature was set at 35 ℃.Results The linear ranges of Notoginsenoside R1 and ginsenoside Rf were 0.198-9.535 μg (r=0.9993) and 0.957-38.392 μg(r=0.9997) respectively.The average recoveries were 99.10％(RSD=1.57％,n=6) and 98.76％(RSD=2.13％,n=6)respectively.Conclusions This HPLC method was accurate,reliable and reproducible,which can contribute to formulating the quality standard of Shenlong Tongluo pills.
关键词
参龙通络丸 /
三七皂苷R1 /
人参皂苷Rf /
高效液相色谱法
Key words
Shenlong Tongluo pills /
Notoginsenoside R1 /
ginsenoside Rf /
HPLC
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 王 晶,张 勇,毕萌菲,等.RP-HPLC法分析测定人参、西洋参、三七中的皂苷类化合物[J].食品研究与开发,2020,41(7):172-176.
[2] Wang Y,Choi H k,Brincmann J A,et al.Chemical analysis of Panax quinquefolius(North American ginseng):a review[J].J Chroma A,2015,1426:1-15.
[3] Zhang X,Gao Y,Zang P,et al.Study on the simultaneous degradation of five pesticides by Paenibacillus polymyxa from Panax ginseng and the characteristics of their products[J].Ecotoxicology and Environmental Safety,2019,168:415-422.
[4] 何耀慧,林荣锋,陈剑平,等.人参、三七在复方芪术调经散中的高效液相色谱法鉴别[J].广州中医药大学学报,2009,26(5):468-470.
[5] 杨 雨.西洋参茎叶皂苷类化学成分的研究[D].北京:中国农业科学院,2014.
[6] 董晓强,董文天,洪 霞,等.三七、人参和西洋参化学成分与药效学之间的关系[J].承德医学院学报,2011,28(3):307-309.
[7] 陈剑平,郑 平,李中桂,等.HPLC-MS法同时测定健脾益肾丸中8个成分的含量[J].药物分析杂志,2017,37(6):962-967.
[8] 张 永,陈 旭,丁 越,等.HPLC法结合统计学方法研究复方丹参片中皂苷类成分[J].辽宁中医杂志,2019,46(1):120-123.
[9] 靳瑞婷,杨素德,付 娟,等.一测多评法测定芪白平肺颗粒中8种皂苷类成分[J].中草药,2015,46(24):3682-3686.
[10] Zhang Jing,Chen Quancheng,Gong Xiaojie,et al.Effect of different method on extracting ratios of ginsenosides[J].J Jilin Agricul University,2003,25(1):71-73.
[11] 宋幸辉,郑宝亮,王长林,等,人参茎叶总皂苷的提取方法研究[J].现代牧业,2017,1(1):30-32.
[12] 翟为民,袁永生,周玉新,等.人参、西洋参及三七参指纹图谱鉴别[J].中国中药杂志,2001,26(7):481.
[13] 张文宗,张 璞.HPLC波长切换法同时测定益安宁丸中7种活性成分[J].中草药,2019,50(8):1947-1951.
[14] 李 宁,高小惠,王子幼,等.三七HPLC指纹图谱及5种成分测定[J].中成药,2020,42(5):1233-1237.
[15] 郭 冲,郜玉钢,臧 埔,等.HPLC法同时测定人参及其制剂中16种人参皂苷[J].中草药,2014,45(14):2009-2013.
PDF(705 KB)
