目的 探讨生长分化因子11(growth differentiation factor 11,GDF1)对高糖环境中人甲状腺乳头状癌K1细胞凋亡的影响。方法 将人甲状腺乳头状癌K1细胞分为3组:对照组(Vehicle)、高糖组(HG)和GDF11组(HG+GDF11;GDF11)。各组细胞培养3 d后,流式细胞术检测细胞凋亡率,western blot检测凋亡相关蛋白Bax与Bcl-2的蛋白水平,实时荧光定量逆转录PCR检测核转录因子E2相关因子(nuclear factor erythroid2-related factor 2,Nrf2)的转录表达,活性氧(ROS)试剂盒检测细胞内的ROS水平。结果 与对照组相比,高糖环境中K1细胞的凋亡率降低,Bcl-2/Bax蛋白表达比升高,Nrf2基因表达上调,细胞内ROS水平下降;而GDF11干预显著提高高糖环境中K1细胞的凋亡率,降低Bcl-2/Bax蛋白表达比,下调Nrf2基因转录表达,并提高细胞内的ROS水平。结论 GDF11可能通过提高高糖环境中K1细胞内的氧化应激反应促进细胞凋亡。
Abstract
Objective To investigate the effects of GDF11 on apoptosis of human papillary thyroid cancer K1 cells under high glucose conditions. Methods Human papillary thyroid cancer K1 cells from the vehicle, HG (high glucose), and GDF11 (HG plus GDF11 administration) groups were cultured for 3 days. Flow cytometry was used to evaluate the apoptotic rate, while Western blot was used to investigate protein levels of Bax and Bcl-2. The expression levels of Nrf2 were determined via qRT-PCR while ROS levels in K1 cells were detected by ROS kit. Results Compared with the vehicle group, the apoptotic rate of HG was decreased, Bcl-2/Bax was increased, expression levels of Nrf2 were up-regulated, and ROS levels were significantly decreased. GDF11 administration increased K1 apoptosis, reduced Bcl-2/Bax protein levels, down-regulated Nrf2 expression levels, and increased ROS levels. Conclusions GDF11 may promote K1 cell apoptosis under high glucose conditions by activating intracellular oxidative stress.
关键词
GDF11 /
糖尿病 /
人甲状腺乳头状癌K1细胞 /
细胞凋亡
Key words
DGF11 /
diabetes /
human papillary thyroid cancer K1 cells /
cell apoptosis
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基金
中国博士后科学基金资助项目(2019T120980)
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